Anti-Thrombin 抗体 (ab92621)
Key features and details
- Rabbit polyclonal to Thrombin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Thrombin antibody
Thrombin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Thrombin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Rabbit, Pig, Macaque monkey, Orangutan
-
免疫原
Synthetic peptide corresponding to Human Thrombin aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102604) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Human Liver Tissue; Mouse Liver Tissue; Rat Liver Tissue; Human Plasma Total Protein; Mouse Plasma
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92621の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 70 kDa).
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
-
機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
細胞内局在
Secreted, extracellular space. - Information by UniProt
-
参照データベース
- Entrez Gene: 2147 Human
- Entrez Gene: 14061 Mouse
- Entrez Gene: 29251 Rat
- Omim: 176930 Human
- SwissProt: P00734 Human
- SwissProt: P19221 Mouse
- SwissProt: P18292 Rat
- Unigene: 655207 Human
see all -
別名
- Coagulation factor II antibody
- Coagulation factor II thrombin antibody
- EC 3.4.21.5 antibody
see all
画像
-
Western blot - Anti-Thrombin antibody (ab92621)All lanes : Anti-Thrombin antibody (ab92621) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : Plasma (Human) Total Protein Lysate
Lane 5 : Plasma (Mouse) Total Protein Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab92621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (17)
ab92621 は 17 報の論文で使用されています。
- Lu Y et al. Pretreatment with Eupatilin Attenuates Inflammation and Coagulation in Sepsis by Suppressing JAK2/STAT3 Signaling Pathway. J Inflamm Res 16:1027-1042 (2023). PubMed: 36926276
- Kim S et al. Control of hippocampal prothrombin kringle-2 (pKr-2) expression reduces neurotoxic symptoms in five familial Alzheimer's disease mice. Br J Pharmacol 179:998-1016 (2022). PubMed: 34524687
- Choi YJ et al. Promotion of the inflammatory response in mid colon of complement component 3 knockout mice. Sci Rep 12:1700 (2022). PubMed: 35105928
- Zhao C et al. Delayed administration of nafamostat mesylate inhibits thrombin-mediated blood-spinal cord barrier breakdown during acute spinal cord injury in rats. J Neuroinflammation 19:189 (2022). PubMed: 35842640
- Yuliani FS et al. Thrombin induces IL-8/CXCL8 expression by DCLK1-dependent RhoA and YAP activation in human lung epithelial cells. J Biomed Sci 29:95 (2022). PubMed: 36369000