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リコンビナントRabMAb

Anti-Thioredoxin 2 抗体 [EPR15225] (ab185544)

Submit a review Submit a question References (27)
Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15225] to Thioredoxin 2
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Thioredoxin 2 antibody [EPR15225]
    Thioredoxin 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15225] to Thioredoxin 2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Raji and HeLa cell lysate. Human liver and mouse kidney tissue. HeLa and K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185544の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

  • 細胞内局在

    Mitochondrial

  • 参照データベース

    • 別名

      • mitochondrial thioredoxin antibody
      • MT-TRX antibody
      • MTRX antibody
      • thioredoxin 2 precursor antibody
      • Thioredoxin mitochondrial antibody
      • Thioredoxin2 antibody
      • TRX 2 antibody
      • TRX2 antibody
      • TXN 2 antibody
      • TXN2 antibody
      see all

    画像

    • Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      All lanes : Anti-Thioredoxin 2 antibody [EPR15225] (ab185544) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 18 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

    • Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. 

       

       

    • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    参考文献 (27)

    ab185544 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185544 は 27 報の論文で使用されています。

    • Kim HJ  et al. scAAV2-Mediated Expression of Thioredoxin 2 and C3 Transferase Prevents Retinal Ganglion Cell Death and Lowers Intraocular Pressure in a Mouse Model of Glaucoma. Int J Mol Sci 24:N/A (2023). PubMed: 38003443
    • Yang X  et al. Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics. Oxid Med Cell Longev 2022:4775645 (2022). PubMed: 35340203
    • Staehr C  et al. Stress adaptation in rats associate with reduced expression of cerebrovascular Kv7.4 channels and biphasic neurovascular responses. Stress 25:227-234 (2022). PubMed: 35666099
    • Han B  et al. Upregulation of PGC-1α Attenuates Oxygen-Glucose Deprivation-Induced Hippocampal Neuronal Injury. Neural Plast 2022:9682999 (2022). PubMed: 35719138
    • Zhang H  et al. Mitochondrial dysfunction induces ALK5-SMAD2-mediated hypovascularization and arteriovenous malformations in mouse retinas. Nat Commun 13:7637 (2022). PubMed: 36496409
    View all Publications for this product

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