Anti-Thioredoxin / TRX 抗体 (ab185329)
Key features and details
- Rabbit polyclonal to Thioredoxin / TRX
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Thioredoxin / TRX antibody
Thioredoxin / TRX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Thioredoxin / TRX -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Thioredoxin/ TRX aa 50 to the C-terminus. (Genbank No.: NP_003320)
Database link: P10599 -
ポジティブ･コントロール
- HeLa and MCF7 cell lysates; Human liver cancer tissue lysate; Human thyroid cancer tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185329の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/600 - 1/5000. Predicted molecular weight: 12 kDa.
|
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| IHC-P | (1) |
1/40 - 1/200.
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| 特記事項 |
|---|
|
WB
1/600 - 1/5000. Predicted molecular weight: 12 kDa. |
|
IHC-P
1/40 - 1/200. |
ターゲット情報
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機能
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). -
配列類似性
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
翻訳後修飾
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. -
細胞内局在
Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7295 Human
- Entrez Gene: 22166 Mouse
- Entrez Gene: 116484 Rat
- Omim: 187700 Human
- SwissProt: P10599 Human
- SwissProt: P10639 Mouse
- SwissProt: P11232 Rat
- Unigene: 435136 Human
see all -
別名
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
see all
画像
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Western blot - Anti-Thioredoxin / TRX antibody (ab185329)All lanes : Anti-Thioredoxin / TRX antibody (ab185329) at 1/600 dilution
Lane 1 : Hela cell tissue lysate
Lane 2 : MCF7 cell tissue lysate
Lane 3 : Human liver cancer tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 12 kDa
Exposure time: 20 seconds12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody (ab185329)Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Thioredoxin / TRX using ab185329 at a 1/40 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185329 は 1 報の論文で使用されています。
- Ma J et al. SBP1 promotes tumorigenesis of thyroid cancer through TXN/NIS pathway. Mol Med 29:121 (2023). PubMed: 37684566