Anti-THEM4 抗体 (ab106435)
Key features and details
- Rabbit polyclonal to THEM4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-THEM4 antibody -
製品の詳細
Rabbit polyclonal to THEM4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
18 amino acid synthetic peptide designed near the center of Human THEM4 (NP_444283).
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ポジティブ･コントロール
- Human liver tissue / tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106435の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 2.5 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
According to PubMed:11598301, inhibits AKT1 phosphorylation and activity. According to PubMed:17615157, enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane. -
組織特異性
Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cell membrane. Cell projection > ruffle membrane. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 117145 Human
- Entrez Gene: 75778 Mouse
- Entrez Gene: 361992 Rat
- Omim: 606388 Human
- SwissProt: Q5T1C6 Human
- SwissProt: Q3UUI3 Mouse
- SwissProt: Q566R0 Rat
- Unigene: 164070 Human
see all -
別名
- Acyl CoA thioesterase THEM4 antibody
- Acyl coenzyme A thioesterase THEM4 antibody
- C terminal modulator protein antibody
see all
画像
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Western blot - Anti-THEM4 antibody (ab106435)Lane 1 : Anti-THEM4 antibody (ab106435) at 1 µg/ml
Lane 2 : Anti-THEM4 antibody (ab106435) at 2 µg/ml
All lanes : Human liver tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 27 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-THEM4 antibody (ab106435)Immunofluorescence of THEM4 in Human liver tissue with ab106435 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEM4 antibody (ab106435)ab106435 at 2.5µg staining THEM4 in paraffin embedded Human liver tissue by immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab106435 は 2 報の論文で使用されています。
- Zhang S et al. Exosomal miR-183-5p Shuttled by M2 Polarized Tumor-Associated Macrophage Promotes the Development of Colon Cancer via Targeting THEM4 Mediated PI3K/AKT and NF-κB Pathways. Front Oncol 11:672684 (2021). PubMed: 34249713
- Li J et al. Age-Related Upregulation of Carboxyl Terminal Modulator Protein Contributes to the Decreased Brain Ischemic Tolerance in Older Rats. Mol Neurobiol 55:6145-6154 (2018). PubMed: 29250714