Anti-THEA 抗体 [EPR18792] (ab180745)
![Western blot - Anti-THEA antibody [EPR18792] (ab180745)](https://www.abcam.co.jp/ps/products/180/ab180745/Images/ab180745-281027-anti-acot11-antibody-epr18792-western-blot.jpg "Western blot - Anti-THEA antibody [EPR18792] (ab180745)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18792] to THEA
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-THEA antibody [EPR18792]
THEA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18792] to THEA -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human fetal liver, heart and kidney tissue lysates; Mouse brown adipose, kidney, heart and spleen tissue lysates; NIH/3T3, Jurkat, LNCaP and HepG2 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18792 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180745の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. -
組織特異性
Isoform 1 is predominantly expressed in skeletal muscle, liver, testis, stomach, spleen, lung and brain. Isoform 2 is predominantly expressed in kidney, uterus, hibernoma and white adipose tissue. -
配列類似性
Contains 2 acyl coenzyme A hydrolase domains.
Contains 1 START domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 26027 Human
- Entrez Gene: 329910 Mouse
- Omim: 606803 Human
- SwissProt: Q8WXI4 Human
- SwissProt: Q8VHQ9 Mouse
- Unigene: 729424 Human
- Unigene: 222956 Mouse
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別名
- ACO11_HUMAN antibody
- ACOT 11 antibody
- ACOT11 antibody
see all
画像
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Western blot - Anti-THEA antibody [EPR18792] (ab180745)All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.
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![Western blot - Anti-THEA antibody [EPR18792] (ab180745)](https://www.abcam.co.jp/ps/products/180/ab180745/Images/ab180745-281028-anti-acot11-antibody-epr18792-western-blot.jpg)
Western blot - Anti-THEA antibody [EPR18792] (ab180745)All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 67,68 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.
This antibody can recognize 2 isoforms in human, the predicted MW is 68 & 67 KDa.
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![Western blot - Anti-THEA antibody [EPR18792] (ab180745)](https://www.abcam.co.jp/ps/products/180/ab180745/Images/ab180745-281029-anti-acot11-antibody-epr18792-western-blot.jpg)
Western blot - Anti-THEA antibody [EPR18792] (ab180745)All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Mouse brown adipose lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 22427358 and PMID:11696000).
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![Western blot - Anti-THEA antibody [EPR18792] (ab180745)](https://www.abcam.co.jp/ps/products/180/ab180745/Images/ab180745-281030-anti-acot11-antibody-epr18792-western-blot.jpg)
Western blot - Anti-THEA antibody [EPR18792] (ab180745)All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse kidney lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 1 minute; Lane 3: 10 seconds; Lane 4: 15 seconds.
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![Anti-THEA antibody [EPR18792] (ab180745)](https://www.abcam.co.jp/ps/products/180/ab180745/Images/ab180745-5-benefits-of-recombinant-antibodies.png)
Anti-THEA antibody [EPR18792] (ab180745)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab180745 は論文での使用が確認できていません。