Anti-THEA 抗体 (ab153835)
Key features and details
- Rabbit polyclonal to THEA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-THEA antibody
THEA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to THEA -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human THEA aa 18-238.
Sequence:R TSRKSALRAG NDSAMADGEG YRNPTEVQMS QLVLPCHTNQ RGELSVGQLL KWIDTTACLS AERHAGCPCV TASMDDIYFE HTISVGQVVN IKAKVNRAFN SSMEVGIQVA SEDLCSEKQW NVCKALATFV ARREITKVKL KQITPRTEEE KMEHSVAAER RRMRLVYADT IKDLLANCAI QGDLESRDCS RMVPAEKTRV ESVELVLPPH ANHQGNTF
Database link: Q8WXI4 -
ポジティブ･コントロール
- 293T, A431, Jurkat cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153835の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
|
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. -
組織特異性
Isoform 1 is predominantly expressed in skeletal muscle, liver, testis, stomach, spleen, lung and brain. Isoform 2 is predominantly expressed in kidney, uterus, hibernoma and white adipose tissue. -
配列類似性
Contains 2 acyl coenzyme A hydrolase domains.
Contains 1 START domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 26027 Human
- Omim: 606803 Human
- SwissProt: Q8WXI4 Human
- Unigene: 729424 Human
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別名
- ACO11_HUMAN antibody
- ACOT 11 antibody
- ACOT11 antibody
see all
画像
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Western blot - Anti-THEA antibody (ab153835)Anti-THEA antibody (ab153835) at 1/1000 dilution + Jurkat 30µg whole cell lysate
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-THEA antibody (ab153835)Immunofluorescence analysis of methanol-fixed A431 using ab153835 antibody at 1/200 dilution. The bottom image shows counterstaining with a nuclear dye.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153835 は 2 報の論文で使用されています。
- Liu KT et al. Regulatory mechanism of fatty acid-CoA metabolic enzymes under endoplasmic reticulum stress in lung cancer. Oncol Rep 40:2674-2682 (2018). PubMed: 30132556
- Hung JY et al. Overexpression and proliferation dependence of acyl-CoA thioesterase 11 and 13 in lung adenocarcinoma. Oncol Lett 14:3647-3656 (2017). PubMed: 28927126