Anti-TGN46 抗体 (ab50595)
Key features and details
- Rabbit polyclonal to TGN46
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TGN46 antibody
TGN46 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TGN46 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human TGN46 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: O43493-2 -
ポジティブ･コントロール
- ICC/IF: Hep G2 and A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50595の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (6) |
Use a concentration of 5 - 10 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
ターゲット情報
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機能
May be involved in regulating membrane traffic to and from trans-Golgi network. -
組織特異性
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells. -
細胞内局在
Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 10618 Human
- Entrez Gene: 192152 Rat
- Omim: 603062 Human
- SwissProt: O43493 Human
- Unigene: 593382 Human
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別名
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)ab50595 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (ab269510). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 0.1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)ab50595 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)This image is courtesy of an abreview submitted by Dr Armen Petrosyan, Univ of Nebraska.Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.
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Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)This image is courtesy of an anonymous Abreviewab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate. -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)This image is courtesy of Alessandro Marcello, Int Ctr Genet Engn & Biotechnol, Italyab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (71)
ab50595 は 71 報の論文で使用されています。
- Nabavizadeh N et al. A progeroid syndrome caused by a deep intronic variant in TAPT1 is revealed by RNA/SI-NET sequencing. EMBO Mol Med 15:e16478 (2023). PubMed: 36652330
- Harwood MC et al. HPV is a cargo for the COPI sorting complex during virus entry. Sci Adv 9:eadc9830 (2023). PubMed: 36662862
- Sørensen DM et al. Identification of global inhibitors of cellular glycosylation. Nat Commun 14:948 (2023). PubMed: 36804936
- Thüring EM et al. Membrane recruitment of the polarity protein Scribble by the cell adhesion receptor TMIGD1. Commun Biol 6:702 (2023). PubMed: 37430142
- Choi J & DiMaio D Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry. PLoS Pathog 19:e1011648 (2023). PubMed: 37703297