Anti-TGFBI 抗体 [EPR17990-13]

• IP

Supplier Data

Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (AB187085)

TGFBI was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab187085 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187085 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse eyeball lysate 10 μg (Input).

Lane 2 : ab187085 IP in mouse eyeball lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187085 in mouse eyeball lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085)

Predicted band size: 75 kDa

false

• WB

Supplier Data

Western blot - Anti-TGFBI antibody [EPR17990-13] (AB187085)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 1 second; Lanes 3 and 4 : 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID : 19478074).

All lanes:

Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085) at 1/1000 dilution

Lane 1:

Rat eyeball lysate at 20 µg

Lane 2:

Mouse eyeball lysate at 20 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat liver lysate at 10 µg

Secondary

Lanes 1, 3 and 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 75 kDa

Observed band size: 68 kDa

true