Anti-TGF beta Receptor II 抗体 [EPR14673]

• WB

Supplier Data

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

ab184948 was shown to react with TGFBR2 in wild-type A549 cells in Western blot with loss of signal observed in TGFBR2 knockout cell line ab261863. Wild-type A549 and TGFBR2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab184948 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/1000 dilution

Lane 1:

Wild-type A549 lysate at 30 µg

Lane 2:

TGFBR2 knock-out A549 at 30 µg

Lane 2:

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line (<a href='/products/cell-lines/human-tgfbr2-tgf-beta-receptor-ii-knockout-a549-cell-line-ab261863'>ab261863</a>)

Lane 2:

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate (<a href='/products/cell-lysates/human-tgfbr2-tgf-beta-receptor-ii-knockout-a549-cell-lysate-ab261672'>ab261672</a>)

Observed band size: 66-95 kDa

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• WB

Lab

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

Lanes 1 - 4 : Merged signal (red and green). Green - ab184948 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab184948 was shown to specifically react with in wild-type A549 cells as signal was lost in TGFBR2 knockout cell line ab261863 (knockout cell lysate ab261672). Wild-type and TGFBR2 knockout samples were subjected to SDS-PAGE. ab184948 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/2000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TGFBR2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line (<a href='/products/cell-lines/human-tgfbr2-tgf-beta-receptor-ii-knockout-a549-cell-line-ab261863'>ab261863</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HT-1080 whole cell lysate at 20 µg

Predicted band size: 65 kDa

Observed band size: 80 kDa

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• WB

Supplier Data

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (ab184948) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 65 kDa

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