Anti-TGF alpha 抗体 (ab9585)
Key features and details
- Rabbit polyclonal to TGF alpha
- Suitable for: ELISA, Neutralising, Sandwich ELISA, WB
- Reacts with: Mouse, Human
- Isotype: unknown
製品の概要
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製品名
Anti-TGF alpha antibody
TGF alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TGF alpha -
由来種
Rabbit -
特異性
The immunogen shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. -
アプリケーション
適用あり: ELISA, Neutralising, Sandwich ELISA, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein aa 1-100.
Database link: P01135 -
特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab208156
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C. -
保存方法
Shipped at 4°C. Store at -20ºC. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α. |
|
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 ug/ml of this antibody is required. |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
組織特異性
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
参照データベース
- Entrez Gene: 7039 Human
- Entrez Gene: 21802 Mouse
- Omim: 190170 Human
- SwissProt: P01135 Human
- SwissProt: P48030 Mouse
- Unigene: 170009 Human
- Unigene: 137222 Mouse
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別名
- EGF like TGF antibody
- EGF-like TGF antibody
- ETGF antibody
see all
画像
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Sandwich ELISA - Anti-TGF alpha antibody (ab9585)
ab9585 detecting TGF-alpha using sELISA
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab9585 は 19 報の論文で使用されています。
- Chiasson-MacKenzie C et al. Cellular mechanisms of heterogeneity in NF2-mutant schwannoma. Nat Commun 14:1559 (2023). PubMed: 36944680
- Wei Y et al. EGFR Signaling Is Required for Maintaining Adult Cartilage Homeostasis and Attenuating Osteoarthritis Progression. J Bone Miner Res 37:1012-1023 (2022). PubMed: 35191092
- Tofteng SS et al. Increased COX-2 after ureter obstruction attenuates fibrosis and is associated with EP2 receptor upregulation in mouse and human kidney. Acta Physiol (Oxf) 235:e13828 (2022). PubMed: 35543087
- Cai W et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice. J Clin Invest 132:N/A (2022). PubMed: 35912857
- Monteiro C et al. Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism. Nat Med 28:752-765 (2022). PubMed: 35411077
