Anti-TGE 抗体 (ab27001)
Key features and details
- Rabbit polyclonal to TGE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TGE antibody
TGE 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TGE -
由来種
Rabbit -
特異性
This antibody is specific to TGE from epidermal cell. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TGE.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/1000. Predicted molecular weight: 76 kDa.
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| 特記事項 |
|---|
|
WB
1/200 - 1/1000. Predicted molecular weight: 76 kDa. |
ターゲット情報
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機能
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. -
配列類似性
Belongs to the transglutaminase superfamily. Transglutaminase family. -
翻訳後修飾
Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). - Information by UniProt
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参照データベース
- Entrez Gene: 7053 Human
- Omim: 600238 Human
- SwissProt: Q08188 Human
- Unigene: 2022 Human
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別名
- E polypeptide protein glutamine gamma glutamyltransferase antibody
- MGC126249 antibody
- MGC126250 antibody
see all
画像
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Western blot - Anti-TGE antibody (ab27001)20 µg of human epidermal lysate was loaded. Following protein transfer ab27001 was applied at a dilution of 1/500. An anti-Rabbit secondary antibody (non-Abcam) was used at 1/2000. Molecular weight 76 kDa.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab27001 は 2 報の論文で使用されています。
- Feng Y et al. TGM3 functions as a tumor suppressor by repressing epithelial-to-mesenchymal transition and the PI3K/AKT signaling pathway in colorectal cancer. Oncol Rep 43:864-876 (2020). PubMed: 32020212
- Zone JJ et al. Dermatitis herpetiformis sera or goat anti-transglutaminase-3 transferred to human skin-grafted mice mimics dermatitis herpetiformis immunopathology. J Immunol 186:4474-80 (2011). IHC-Fr ; Mouse . PubMed: 21335491
