Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-TFPI2 抗体 [EPR14442] - BSA and Azide free (ab246362)

Submit a review Submit a question
Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)
  • Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14442] to TFPI2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TFPI2 antibody [EPR14442] - BSA and Azide free
    TFPI2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14442] to TFPI2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human kidney tissue sections. WB: Jurkat, MCF7, rat spleen, HeLa, mouse heart, mouse brain, mouse kidney and mouse spleen lysates. IP: TFPI2 IP in HeLa cell lysate.

  • 特記事項

    ab246362 is the carrier-free version of ab186747.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246362の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

  • 組織特異性

    Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.

  • 配列類似性

    Contains 3 BPTI/Kunitz inhibitor domains.

  • ドメイン

    This inhibitor contains three inhibitory domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Placental protein 5 antibody
      • PP5 antibody
      • REF1 antibody
      • Retinal pigment epithelium cell factor 1 antibody
      • TFPI 2 antibody
      • TFPI-2 antibody
      • TFPI2 antibody
      • TFPI2 PEN antibody
      • TFPI2_HUMAN antibody
      • Tissue factor pathway inhibitor 2 antibody
      see all

    画像

    • Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)
      Immunoprecipitation - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)

      Lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab186747).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)

      Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab186747).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)
      Anti-TFPI2 antibody [EPR14442] - BSA and Azide free (ab246362)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab246362 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab246362 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab246362.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com