Anti-TFIIE alpha/GTF2E1 抗体 (ab28177)
Key features and details
- Rabbit polyclonal to TFIIE alpha/GTF2E1
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TFIIE alpha/GTF2E1 antibody
TFIIE alpha/GTF2E1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TFIIE alpha/GTF2E1 -
由来種
Rabbit -
特異性
ab28177 recognises TFIIE alpha/GTF2E1.
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アプリケーション
適用あり: ChIPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human TFIIE alpha/GTF2E1.
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ポジティブ・コントロール
- HeLa nuclear extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.9
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28177の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP | (1) |
Use 5-10 µg for 25 µg of chromatin.
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特記事項 |
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ChIP
Use 5-10 µg for 25 µg of chromatin. |
ターゲット情報
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機能
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. -
配列類似性
Belongs to the TFIIE alpha subunit family.
Contains 1 HTH TFE/IIEalpha-type domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2960 Human
- Omim: 189962 Human
- SwissProt: P29083 Human
- Unigene: 445272 Human
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別名
- FE antibody
- General transcription factor IIE 56 kDa subunit antibody
- General transcription factor IIE subunit 1 antibody
see all
画像
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Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab28177 は 1 報の論文で使用されています。
- Petkau N et al. Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. Elife 8:N/A (2019). PubMed: 31294688