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AB109106

Anti-TFIIB 抗体 [EPR4589]

Anti-TFIIB antibody [EPR4589]

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(3 Publications)

Rabbit Recombinant Monoclonal TFIIB antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

別名を表示する

TF2B, TFIIB, GTF2B, Transcription initiation factor IIB, General transcription factor TFIIB, S300-II

1 Images
Western blot - Anti-TFIIB antibody [EPR4589] (AB109106)
  • WB

Unknown

Western blot - Anti-TFIIB antibody [EPR4589] (AB109106)

All lanes:

Western blot - Anti-TFIIB antibody [EPR4589] (ab109106) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

PC12 cell lysate at 10 µg

Predicted band size: 34 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-TFIIB antibody [EPR4589] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4589

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed : 12931194, PubMed : 1517211, PubMed : 1876184, PubMed : 1946368, PubMed : 27193682, PubMed : 3029109, PubMed : 3818643, PubMed : 7601352, PubMed : 8413225, PubMed : 8515820, PubMed : 8516311, PubMed : 8516312, PubMed : 9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed : 8413225, PubMed : 8504927, PubMed : 8515820, PubMed : 8516311, PubMed : 8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed : 7601352). Associates with chromatin to core promoter-specific regions (PubMed : 12931194, PubMed : 24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed : 10619841, PubMed : 16230532, PubMed : 7675079, PubMed : 9420329). Modulates transcription start site selection (PubMed : 10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed : 12931194).
See full target information GTF2B
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Endocrine journal 69:1117-1129 PubMed35545536

2022

Overexpression of lncRNA HCP5 in human umbilical cord mesenchymal stem cell-derived exosomes promoted the proliferation and inhibited the apoptosis of ovarian granulosa cells via the musashi RNA-binding protein 2/oestrogen receptor alpha 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ting Sun,Jun-Hong Cai,Shan Bao

Translational oncology 14:100947 PubMed33227664

2020

Overexpression of long non-coding RNA00355 enhances proliferation, chemotaxis, and metastasis in colon cancer via promoting GTF2B-mediated ITGA2.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Ruan,Hongling Deng,Minhua Liang,Zhe Xu,Manxiang Lai,Hong Ren,Xiangliang Deng,Xinguo Su

Nature communications 7:13710 PubMed27966531

2016

Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment.

Applications

WB

Species

Unspecified reactive species

Jin Yang,Konstantinos Savvatis,Jong Seok Kang,Peidong Fan,Hongyan Zhong,Karen Schwartz,Vivian Barry,Amanda Mikels-Vigdal,Serge Karpinski,Dmytro Kornyeyev,Joanne Adamkewicz,Xuhui Feng,Qiong Zhou,Ching Shang,Praveen Kumar,Dillon Phan,Mario Kasner,Begoña López,Javier Diez,Keith C Wright,Roxanne L Kovacs,Peng-Sheng Chen,Thomas Quertermous,Victoria Smith,Lina Yao,Carsten Tschöpe,Ching-Pin Chang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB109518

Anti-TFIIB antibody [EP4588]

primary-antibodies

tfiib-antibody-ep4588-ab109518

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