Anti-TFIIB 抗体 [EP4588] (ab109518)
![Western blot - Anti-TFIIB antibody [EP4588] (ab109518)](https://www.abcam.co.jp/ps/products/109/ab109518/Images/ab109518-330939-anti-tfiib-antibody-ep4588-western-blot.jpg "Western blot - Anti-TFIIB antibody [EP4588] (ab109518)")
Key features and details
- Rabbit monoclonal [EP4588] to TFIIB
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-TFIIB antibody [EP4588]
TFIIB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP4588] to TFIIB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-P, Flow Cyt (Intra)more details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2, HeLa, Jurkat and K562 cell lysates; IHC-P: Human breast Tissue IP: Jurkat cells Flow Cyt (intra): Jurkat
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP4588 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109518の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
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IP
1/10 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. -
配列類似性
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 81673 Rat
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P62916 Rat
- Unigene: 481852 Human
see all -
別名
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
see all
画像
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Western blot - Anti-TFIIB antibody [EP4588] (ab109518)All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa -
![Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)](https://www.abcam.co.jp/ps/products/109/ab109518/Images/ab109518-330905-anti-tfiib-antibody-ep4588-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TFIIB antibody [EP4588] (ab109518)ab109518 (purified) at 1/20 dilution (0.16 µg/ml) immunoprecipitating TFIIB in Jurkat whole cell lysate .
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab109518 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109518 in Jurkat whole cell lysate.
For western blotting, ab109518 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
![Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (ab109518)](https://www.abcam.co.jp/ps/products/109/ab109518/Images/ab109518-330896-anti-tfiib-antibody-ep4588-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TFIIB antibody [EP4588] (ab109518)Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling TFIIB with ab109518 at 1/20 dilution (4.05 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
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![Western blot - Anti-TFIIB antibody [EP4588] (ab109518)](https://www.abcam.co.jp/ps/products/109/ab109518/Images/ab109518-122433-anti-tfiib-antibody-ep4588-western-blot.jpg)
Western blot - Anti-TFIIB antibody [EP4588] (ab109518)All lanes : Anti-TFIIB antibody [EP4588] (ab109518) at 1/1000 dilution
Lane 1 : HepG2 cell lyaste
Lane 2 : HeLa cell lyaste
Lane 3 : Jurkat cell lyaste
Lane 4 : K562 cell lyaste
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518)](https://www.abcam.co.jp/ps/products/109/ab109518/Images/ab109518-122432-anti-tfiib-antibody-ep4588-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFIIB antibody [EP4588] (ab109518)ab109518, at a 1/100 dilution, staining TFIIB in paraffin embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab109518 は論文での使用が確認できていません。