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    products/primary-antibodies/tfii-i-phospho-y248-antibody-ab63344.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription Other
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Anti-TFII I (phospho Y248) 抗体 (ab63344)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Western blot - Anti-TFII I (phospho Y248) antibody (ab63344)

    Key features and details

    • Rabbit polyclonal to TFII I (phospho Y248)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    関連製品

    製品の概要

    • 製品名

      Anti-TFII I (phospho Y248) antibody
      TFII I 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to TFII I (phospho Y248)
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat
    • 免疫原

      Synthetic peptide corresponding to Human TFII I aa 200-300 (phospho Y248).
      Database link: P78347

    • ポジティブ・コントロール

      • Extracts from LOVO cells
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Polymerase associated factors
      • Pol II Transcription
      • Other
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Preinitiation complex

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab63344の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).

    ターゲット情報

    • 機能

      Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
    • 組織特異性

      Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
    • 関連疾患

      Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 配列類似性

      Belongs to the TFII-I family.
      Contains 6 GTF2I-like repeats.
    • 翻訳後修飾

      Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
      Sumoylated.
    • 細胞内局在

      Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
    • Target information above from: UniProt accession P78347 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 2969 Human
      • Entrez Gene: 14886 Mouse
      • Entrez Gene: 353256 Rat
      • Omim: 601679 Human
      • SwissProt: P78347 Human
      • SwissProt: Q9ESZ8 Mouse
      • SwissProt: Q5U2Y1 Rat
      • Unigene: 647041 Human
      • Unigene: 261570 Mouse
      • Unigene: 412191 Mouse
      • Unigene: 27575 Rat
      see all
    • 別名

      • BAP 135 antibody
      • BAP-135 antibody
      • BAP135 antibody
      • Bruton tyrosine kinase associated protein 135 antibody
      • Bruton tyrosine kinase-associated protein 135 antibody
      • BTK associated protein 135 antibody
      • BTK associated protein 135kD antibody
      • BTK associated protein antibody
      • BTK-associated protein 135 antibody
      • BTKAP 1 antibody
      • BTKAP1 antibody
      • DIWS antibody
      • FLJ38776 antibody
      • FLJ56355 antibody
      • General transcription factor II i antibody
      • General transcription factor II-I antibody
      • General transcription factor IIi antibody
      • GTF 2I antibody
      • Gtf2i antibody
      • GTF2I_HUMAN antibody
      • GTFII I antibody
      • GTFII-I antibody
      • IB 291 antibody
      • IB291 antibody
      • SPIN antibody
      • SRF Phox 1 interacting protein antibody
      • SRF Phox1 interacting protein antibody
      • SRF-Phox1-interacting protein antibody
      • TFII-I antibody
      • Transcription factor II I antibody
      • WBS antibody
      • WBSCR 6 antibody
      • WBSCR6 antibody
      • Williams Beuren syndrome chromosome region 6 antibody
      • Williams Beuren syndrome chromosome region 6 protein antibody
      • Williams-Beuren syndrome chromosomal region 6 protein antibody
      see all

    画像

    • Western blot - Anti-TFII I (phospho Y248) antibody (ab63344)
      Western blot - Anti-TFII I (phospho Y248) antibody (ab63344)
      All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution

      Lane 1 : extracts from LOVO cells, without immunising peptide
      Lane 2 : extracts from LOVO cells, with immunising peptide

      Predicted band size: 112 kDa
      Observed band size: 112 kDa
      Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.



      The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (1)

    ab63344 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab63344 は 1 報の論文で使用されています。

    • Gui F  et al. A non-covalent inhibitor XMU-MP-3 overrides ibrutinib-resistant BtkC481S mutation in B-cell malignancies. Br J Pharmacol 176:4491-4509 (2019). PubMed: 31364164

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    We recently bought #ab63344 antibody which recommended for ELISA and WB. We are planning to use the antibody for IP. Could you recommend any positive control for that?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2011

    Answer

    Thank you for contacting us. The antibody ab63344 detects TFII I when phosphorylated at tyrosine 248. We do not have a phosphorylated TFII I protein. We do have a ab114787, which is a non-phosphorylated fragment of TFII I, amino acids 1-274, but this would only serve as a negative control. We do not expect that ab63344 will recognize it. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2011

    Question

    Phone call regarding obtaining testing discounts for untested applications for ab63344 (for IP) and ab80547 (for ICC/IHP) and reporting a complaint in regards to ab80547 with use in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2011

    Answer

    Thank you for contacting us and considering the participation in the testing discount scheme. These are the discount codes for the respective antibodies: ab63344 xxxxxxx ab80547 xxxxxxx Both are valid until the xth of xxx 20xx These codes will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP with ab63344 and ICC/IHP with ab80547 and include the respective code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. More terms and conditions relating to this offer can be found here: www.abcam.com/collaborationdiscount In regards to the problems you have been having in performing Western blotting with ab80547, I have attached a questionnaire for you to complete. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly, and the information from this questionnaire will help greatly in this process. This information will also allow us to see if there are any suggestions we can make that may improve the results. As this antibody (as with all the products in our catalogue) is covered by the Abpromise guarantee, if the product is not working in a satisfactory way in a tested species and application we will provide a free of charge replacement with a different lot or primary antibody, or a full refund. More information on the Abpromise can be found here: www.abcam.com/abpromise

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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