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リコンビナントRabMAb

Anti-TFII I 抗体 [EPR7696] - BSA and Azide free (ab185640)

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Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
  • OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
  • Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7696] to TFII I - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TFII I antibody [EPR7696] - BSA and Azide free
    TFII I 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7696] to TFII I - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal brain lysates; Jurkat, U937, HeLa and 293T cell lysates; Human colon and kidney tissues

  • 特記事項

    ab185640 is the carrier-free version of ab134133.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185640の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

  • 組織特異性

    Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.

  • 関連疾患

    Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

  • 配列類似性

    Belongs to the TFII-I family.
    Contains 6 GTF2I-like repeats.

  • 翻訳後修飾

    Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
    Sumoylated.

  • 細胞内局在

    Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • BAP 135 antibody
    • BAP-135 antibody
    • BAP135 antibody
    • Bruton tyrosine kinase associated protein 135 antibody
    • Bruton tyrosine kinase-associated protein 135 antibody
    • BTK associated protein 135 antibody
    • BTK associated protein 135kD antibody
    • BTK associated protein antibody
    • BTK-associated protein 135 antibody
    • BTKAP 1 antibody
    • BTKAP1 antibody
    • DIWS antibody
    • FLJ38776 antibody
    • FLJ56355 antibody
    • General transcription factor II i antibody
    • General transcription factor II-I antibody
    • General transcription factor IIi antibody
    • GTF 2I antibody
    • Gtf2i antibody
    • GTF2I_HUMAN antibody
    • GTFII I antibody
    • GTFII-I antibody
    • IB 291 antibody
    • IB291 antibody
    • SPIN antibody
    • SRF Phox 1 interacting protein antibody
    • SRF Phox1 interacting protein antibody
    • SRF-Phox1-interacting protein antibody
    • TFII-I antibody
    • Transcription factor II I antibody
    • WBS antibody
    • WBSCR 6 antibody
    • WBSCR6 antibody
    • Williams Beuren syndrome chromosome region 6 antibody
    • Williams Beuren syndrome chromosome region 6 protein antibody
    • Williams-Beuren syndrome chromosomal region 6 protein antibody
    see all

画像

  • Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    Western blot - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    All lanes : Anti-TFII I antibody [EPR7696] (ab134133) at 1/10000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 112 kDa



    This data was developed using ab134133, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    This data was developed using ab134133, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TFII I with ab134133 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    This data was developed using ab134133, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TFII I with ab134133 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    OI-RD Scanning - Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    This data was developed using ab134133, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)
    Anti-TFII I antibody [EPR7696] - BSA and Azide free (ab185640)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab185640 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185640 は論文での使用が確認できていません。

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