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リコンビナントRabMAb

Anti-TFEC 抗体 [EPR14871] (ab185226)

Submit a review Submit a question References (2)
Western blot - Anti-TFEC antibody [EPR14871] (ab185226)
  • Immunoprecipitation - Anti-TFEC antibody [EPR14871] (ab185226)
  • Anti-TFEC antibody [EPR14871] (ab185226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14871] to TFEC
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TFEC antibody [EPR14871]
    TFEC 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14871] to TFEC

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T and HepG2 whole cell lysate (ab7900).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185226の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/40 - 1/60.
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
特記事項
IP
1/40 - 1/60.
WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

ターゲット情報

  • 機能

    Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter (By similarity). Collaborates with MITF in target gene activation (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form.

  • 組織特異性

    Expressed moderately in spleen, kidney, bone marrow, small intestine and leukocytes. Expressed weakly in testis, trachea and colon.

  • 配列類似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • bHLHe34 antibody
      • Class E basic helix-loop-helix protein 34 antibody
      • hTFEC-L antibody
      • TCFEC antibody
      • TFE-C antibody
      • Tfec antibody
      • TFEC protein antibody
      • TFEC_HUMAN antibody
      • TFECL antibody
      • Transcription factor EC antibody
      • Transcription factor EC-like antibody
      see all

    画像

    • Western blot - Anti-TFEC antibody [EPR14871] (ab185226)
      Western blot - Anti-TFEC antibody [EPR14871] (ab185226)
      All lanes : Anti-TFEC antibody [EPR14871] (ab185226) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa

    • Immunoprecipitation - Anti-TFEC antibody [EPR14871] (ab185226)
      Immunoprecipitation - Anti-TFEC antibody [EPR14871] (ab185226)

      Western blot analysis of immunoprecipitation pellet from 293T cell lysate immunoprecipitated using ab185226 at 1/50 dilution.
      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    • Anti-TFEC antibody [EPR14871] (ab185226)
      Anti-TFEC antibody [EPR14871] (ab185226)

    参考文献 (2)

    ab185226 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185226 は 2 報の論文で使用されています。

    • Chen YP  et al. Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma. Cell Res 30:1024-1042 (2020). PubMed: 32686767
    • Liu Y  et al. Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia. Autophagy 15:493-509 (2019). PubMed: 30304977

    レビューと Q&A

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