Anti-TFE3 抗体 (ab245454)
Key features and details
- Rabbit polyclonal to TFE3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TFE3 antibody
TFE3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TFE3 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TFE3 aa 450-500. The exact sequence is proprietary. NP_006512.2
Database link: P19532 -
ポジティブ･コントロール
- IP: TFE3 IP in HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245454 was affinity purified using an epitope specific to TFE3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. -
組織特異性
Ubiquitous in fetal and adult tissues. -
関連疾患
Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. -
配列類似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Sumoylated; does not affect dimerization with MITF. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7030 Human
- Omim: 314310 Human
- SwissProt: P19532 Human
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別名
- bHLH e33 antibody
- bHLHe33 antibody
- Class E basic helix-loop-helix protein 33 antibody
see all
画像
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Immunoprecipitation - Anti-TFE3 antibody (ab245454)
TFE3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245454 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different Anti-TFE3 antibody at 1 µg/ml.
Lane 1: ab245454 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245454 は論文での使用が確認できていません。