Anti-TFB2M 抗体 (ab118321)
Key features and details
- Goat polyclonal to TFB2M
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-TFB2M antibody
TFB2M 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TFB2M -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to Mouse TFB2M aa 50-150 (internal sequence) (Cysteine residue).
Database link: NP_032275.2 -
ポジティブ･コントロール
- Mouse and Rat lung lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118321 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 289307 Rat
- SwissProt: Q5U2T7 Rat
- Unigene: 96104 Rat
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別名
- N''-adenosyl(rRNA) dimethyltransferase 2 antibody
- S adenosylmethionine 6 N', N' adenosyl(rRNA) dimethyltransferase 2 antibody
- Dimethyladenosine transferase 2 antibody
see all
画像
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Western blot - Anti-TFB2M antibody (ab118321)Anti-TFB2M antibody (ab118321) at 0.5 µg/ml + Rat Lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab118321 は論文での使用が確認できていません。