Anti-TFB1M 抗体 (ab236901)
Key features and details
- Rabbit polyclonal to TFB1M
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TFB1M antibody -
製品の詳細
Rabbit polyclonal to TFB1M -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cynomolgus monkey, Orangutan
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免疫原
Recombinant fragment corresponding to Human TFB1M aa 50-200.
Database link: Q8WVM0 -
ポジティブ･コントロール
- WB: A549 whole cell lysate. IHC-P: Human pancreatic cancer and liver cancer tissues. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/5000. Predicted molecular weight: 39 kDa.
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| IHC-P |
1/500 - 1/1000.
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| ICC/IF |
1/50 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/5000. Predicted molecular weight: 39 kDa. |
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IHC-P
1/500 - 1/1000. |
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ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. -
組織特異性
Ubiquitously expressed. -
関連疾患
Note=Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA. -
配列類似性
Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 102130743 Cynomolgus monkey
- Entrez Gene: 51106 Human
- Entrez Gene: 100173901 Orangutan
- Omim: 607033 Human
- SwissProt: Q2PG46 Cynomolgus monkey
- SwissProt: Q8WVM0 Human
- SwissProt: Q5R4V9 Orangutan
- Unigene: 279908 Human
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別名
- N'-adenosyl(rRNA) dimethyltransferase 1 antibody
- CGI75 antibody
- Dimethyladenosine transferase 1, mitochondrial antibody
see all
画像
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Western blot - Anti-TFB1M antibody (ab236901)Anti-TFB1M antibody (ab236901) at 1/500 dilution + A549 (Human lung carcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFB1M antibody (ab236901)Paraffin-embedded human pancreatic cancer tissue stained for TFB1M using ab236901 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFB1M antibody (ab236901)Paraffin-embedded human liver cancer tissue stained for TFB1M using ab236901 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-TFB1M antibody (ab236901)HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for TFB1M (green) using ab236901 at 1/166 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab236901 は 1 報の論文で使用されています。
- Yang W et al. Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1. J Exp Clin Cancer Res 40:92 (2021). PubMed: 33691743