AB34733

Texas Red ® Anti-GST 抗体

Name: Texas Red ® Anti-GST 抗体 (ab34733) | Abcam
Brand: Abcam Limited
SKU: AB34733
Price: 76000 JPY
Availability: InStock

Texas Red ® Anti-GST antibody

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(1 Publication)

Goat Polyclonal GST antibody - conjugated to Texas Red®. Suitable for WB and reacts with Tag samples. Cited in 1 publication.

別名を表示する

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

Supplier Data

Western blot - Texas Red ® Anti-GST antibody (AB34733)

Blocking Buffer for Fluorescent Western Blotting for 30 minutes at room temperature.

All lanes:

Western blot - Texas Red ® Anti-GST antibody (ab34733) at 1/1000 dilution

All lanes:

GST at 0.05 µg

Predicted band size: 25 kDa

Observed band size: 25 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

標識

Texas Red®

励起波長/蛍光波長

Ex: 589nm, Em: 615nm

キャリアフリー

アプリケーション

applications

特異性

Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons). Fluorochrome/Protein Ratio: 2.0 moles Texas Red™ per mole of Goat IgG. Absorption Wavelength: 596 nm; Emission Wavelength: 620 nm.

Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 9:1192-4 PubMed23142872

2012

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Lance Martin,Matthias Meier,Shawn M Lyons,Rene V Sit,William F Marzluff,Stephen R Quake,Howard Y Chang

View all publications

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