Texas Red ® Anti-R Phycoerythrin/rpeA 抗体 (ab34734)
Key features and details
- Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: WB, Dot blot
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Isotype: IgG
製品の概要
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製品名
Texas Red ® Anti-R Phycoerythrin/rpeA antibody
R Phycoerythrin/rpeA 一次抗体 製品一覧 -
製品の詳細
Texas Red ® Goat polyclonal to R Phycoerythrin/rpeA -
由来種
Goat -
標識
Texas Red ®. Ex: 596nm, Em: 620nm -
特異性
This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
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アプリケーション
適用あり: WB, Dot blotmore details -
種交差性
交差種: Red Algae -
免疫原
Full length protein corresponding to R Phycoerythrin/rpeA. This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
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特記事項
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34734の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. -
細胞内局在
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome. -
別名
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
画像
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Texas Red ® Anti-R Phycoerythrin/rpeA antibody (ab34734) at 1/1000 dilution (60 minutes at room temperature) + R-Phycoerythrin at 0.05 µg
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking buffer for fluorescent Western blotting used for 30 minutes at room temperature.
Predicted size: 18 kDa for R-Phycoerythrin.
Other bands: R-Phycoerythrin splice varients and isoforms.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab34734 は 2 報の論文で使用されています。
- Andersen TK et al. Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief. Front Immunol 12:747032 (2021). PubMed: 34691056
- Andersen TK et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines 4:9 (2019). PubMed: 30775000