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Anti-Tetanus toxoid 抗体 [18.2.12.6] - BSA and Azide free (ab300090)

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Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
  • Indirect ELISA - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
  • Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [18.2.12.6] to Tetanus Toxoid - BSA and Azide free
  • Suitable for: WB, Indirect ELISA

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free
    Tetanus Toxoid 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [18.2.12.6] to Tetanus Toxoid - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, Indirect ELISAmore details

  • 種交差性

    交差種: Clostridium tetani

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with Tetanus toxoid expression vector containing a myc-His-tag®, whole cell lysate Indirect ELISA: Tetanus toxoid.

  • 特記事項

    ab300090 is a carrier free version of ab300089. 

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    18.2.12.6

  • アイソタイプ

    IgG2b

  • 研究分野

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300090の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 150,100 kDa (predicted molecular weight: 151 kDa).
Indirect ELISA
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 150,100 kDa (predicted molecular weight: 151 kDa).
Indirect ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Tetanus toxoid is derived from the toxin released by Clostridium Tetanis that causes the disease tetanus. It is used as a vaccine to prevent tetanus or to help boost the immune response to other vaccines. A toxoid is a substance that has been treated to destroy its toxic properties but that retains the capacity to stimulate production of antitoxins, used in immunization. Tetanus toxoid is produced by formaldehyde inactivation of pure neuro toxin. Each lot is verified to be non toxic. In addition to being a potent antigen, tetanus toxoid is a useful carrier protein for making polysaccharides and haptens immunogenic.

  • 細胞内局在

    Secreted

  • 別名

    • Clostridium tetani toxoid antibody
    • Tentoxylysin antibody
    • Tetanus toxin antibody
    • Tetanus toxin tetX antibody
    • tetX antibody
    see all

画像

  • Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
    Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
    All lanes : Anti-Tetanus toxoid antibody [18.2.12.6] (ab300089) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with Tetanus toxoid expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 151 kDa
    Observed band size: 150, 100 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab300089, the same antibody clone in a different buffer formulation. 

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The 100 kDa band is expected to be the cleaved heavy chain. 
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10924793).

  • Indirect ELISA - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
    Indirect ELISA - Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)

    This data was developed using ab300089, the same antibody clone in a different buffer formulation. 

    Indirect ELISA analysis of Tetanus toxoid at 5000 ng/ml with ab300089 at concentration range 1000 - 0 ng/mL. An alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution was used as the secondary antibody.

  • Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)
    Anti-Tetanus toxoid antibody [18.2.12.6] - BSA and Azide free (AB300090)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300090 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300090 は論文での使用が確認できていません。

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