Anti-Tetanus toxoid 抗体 [18.2.12.6] (ab300089)
![Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)](https://www.abcam.co.jp/ps/products/300/ab300089/Images/AB300089-490128-anti-tetanus-toxoid-antibody-18-2-12-60-western-blot-hek-293t.jpg "Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [18.2.12.6] to Tetanus Toxoid
- Suitable for: WB, Indirect ELISA
Related conjugates and formulations
製品の概要
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製品名
Anti-Tetanus toxoid antibody [18.2.12.6]
Tetanus Toxoid 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [18.2.12.6] to Tetanus Toxoid -
由来種
Mouse -
アプリケーション
適用あり: WB, Indirect ELISAmore details -
種交差性
交差種: Clostridium tetani -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HEK-293T transfected with Tetanus toxoid expression vector containing a myc-His-tag®, whole cell lysate Indirect ELISA: Tetanus toxoid.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
18.2.12.6 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 150,100 kDa (predicted molecular weight: 151 kDa).
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| Indirect ELISA |
Use at an assay dependent concentration.
Use at 31.25 ng/ml |
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 150,100 kDa (predicted molecular weight: 151 kDa). |
|
Indirect ELISA
Use at an assay dependent concentration. Use at 31.25 ng/ml |
ターゲット情報
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関連性
Tetanus toxoid is derived from the toxin released by Clostridium Tetanis that causes the disease tetanus. It is used as a vaccine to prevent tetanus or to help boost the immune response to other vaccines. A toxoid is a substance that has been treated to destroy its toxic properties but that retains the capacity to stimulate production of antitoxins, used in immunization. Tetanus toxoid is produced by formaldehyde inactivation of pure neuro toxin. Each lot is verified to be non toxic. In addition to being a potent antigen, tetanus toxoid is a useful carrier protein for making polysaccharides and haptens immunogenic. -
細胞内局在
Secreted -
別名
- Clostridium tetani toxoid antibody
- Tentoxylysin antibody
- Tetanus toxin antibody
see all
画像
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Western blot - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)All lanes : Anti-Tetanus toxoid antibody [18.2.12.6] (ab300089) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with Tetanus toxoid expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 151 kDa
Observed band size: 150, 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The 100 kDa band is expected to be the cleaved heavy chain.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10924793). -
![Indirect ELISA - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)](https://www.abcam.co.jp/ps/products/300/ab300089/Images/AB300089-490129-anti-tetanus-toxoid-antibody-18-2-12-60-indirect-elisa-tetanus-toxoid.jpg)
Indirect ELISA - Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)Indirect ELISA analysis of Tetanus toxoid at 5000 ng/ml with ab300089 at concentration range 1000 - 0 ng/mL. An alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution was used as the secondary antibody.
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![Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)](https://www.abcam.co.jp/ps/products/300/ab300089/Images/AB300089-490126-Benefits-of-Recombinant-antibodies.jpg)
Anti-Tetanus toxoid antibody [18.2.12.6] (AB300089)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab300089 は論文での使用が確認できていません。