Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Tetanus Toxin 抗体 (ab53829)

Submit a review Q&A (3)References (4)

Key features and details

  • Rabbit polyclonal to Tetanus Toxin
  • Suitable for: WB, ELISA
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Tetanus Toxin antibody
    Tetanus Toxin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Tetanus Toxin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Clostridium tetani

  • 免疫原

    Tissue, cells or virus corresponding to Tetanus Toxin. Tetanus toxoid (formaldehyde inactivated tetanus toxin).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab53829の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 150 kDa.
ELISA
Use at an assay dependent dilution.
特記事項
WB
Use at an assay dependent dilution. Predicted molecular weight: 150 kDa.
ELISA
Use at an assay dependent dilution.

ターゲット情報

  • 関連性

    Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

  • 細胞内局在

    Secreted

  • 別名

    • Tentoxylysin antibody
    • Tetanus toxin chain H antibody
    • Tetanus toxin chain L antibody
    • Tetanus toxin heavy chain antibody
    • Tetanus toxin light chain antibody
    see all

参考文献 (4)

ab53829 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab53829 は 4 報の論文で使用されています。

  • Stark JC  et al. Rapid biosynthesis of glycoprotein therapeutics and vaccines from freeze-dried bacterial cell lysates. Nat Protoc 18:2374-2398 (2023). PubMed: 37328605
  • Hodgins HP  et al. Ancient Clostridium DNA and variants of tetanus neurotoxins associated with human archaeological remains. Nat Commun 14:5475 (2023). PubMed: 37673908
  • Chai P  et al. The recombinant protein combined vaccine based on the fragment C of tetanus toxin and the cross-reacting material 197. Appl Microbiol Biotechnol 105:1683-1692 (2021). PubMed: 33511443
  • Mittal AM  et al. Multiple network properties overcome random connectivity to enable stereotypic sensory responses. Nat Commun 11:1023 (2020). PubMed: 32094345

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

I am going to do WB against recombinant universal T-helper cell epitope, callad tt830-844: a.a. qyikanskfigite. I think this three Ab (ab53829, ab68494 and ab35171) can work. Can you confirm if you used the whole protein to induce the imune response? If these are not suitable can you please recommend one.

Please let me know if you require any further information.

Thanks

Kind regards,

Read More
Answer

Thank you for your enquiry.

The immunogen information is published on each of the product datasheets unless it is a commercially sensitive information. Could I advise you and your customer to read the on-line documents to check the tested applications as well.

ab35171: Synthetic peptide conjugated to KLH, corresponding to amino acids 1300-1314 of Tetanus Toxoid (Clostridium Tetani).

ab53829: Tetanus toxoid (formaldehyde inactivated tetanus toxin).

ab68494: Purified toxin.

These tree antibodies are polyclonals so they recognize multiple epitopes. Therefore, fo rpolyclonals epitop mappig is not performed. If the epitope sequence for monoclonal antibodies are mapped then the information is listed on the datasheets under the "Epitope" section.

I hope this will be useful for you.

Read More

Question

MSDS for ab53829.

Read More
Answer

Thank you for calling Abcam. Please find attached the MSDS for ab53829. If there is anything else I can help you with, please let me kn

Read More

Question

I have a customer with the following questions: Item 1 - Ab53589 Goat polyclonal to Tetanus Toxin. - Does this antibody react with the entire toxin or just the C-fragment? Item 2. Ab53829 Rabbit polyclonal to Tetanus Toxin. - What concentration is recommended for WB and ELISA? (not listed on data sheet - but would it be similar to the ab53589?) - I realise they are different concentrations.

Read More
Answer

Thank you for your enquiry. I can confirm that ab53589 can detect the tetanus toxin which is produced by the soil micro-organism Clostridium tetani, and also the C-fragment of tetanus toxin. Meanwhile, for ab53829 ELISA and WB recommend starting dilution, please try 1/500. However, please bear in mind that the optimal dilutions/concentrations should be determined by the end user. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com