Anti-TET3 抗体 (ab153724)
Key features and details
- Rabbit polyclonal to TET3
- Suitable for: ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TET3 antibody
TET3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TET3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1456-1660 of Human TET3 (Uniprot ID: O43151).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153724の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| IP |
1/500 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
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IP
1/500 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). -
組織特異性
Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. -
配列類似性
Belongs to the TET family. -
発生段階
Expressed in fetal brain but not adult brain. - Information by UniProt
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参照データベース
- Entrez Gene: 200424 Human
- Entrez Gene: 194388 Mouse
- Omim: 613555 Human
- SwissProt: O43151 Human
- SwissProt: Q8BG87 Mouse
- Unigene: 516107 Human
- Unigene: 211030 Mouse
- Unigene: 485069 Mouse
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別名
- hCG_40738 antibody
- KIAA0401 antibody
- Methylcytosine dioxygenase TET3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET3 antibody - C-terminal (ab153724)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining TET3 protein at nucleus with ab153724 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-TET3 antibody - C-terminal (ab153724)Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling TET3 protein at cytoplasm and nucleus (green) with ab153724 at 1/400 dilution. Red: phalloidin, a cytoskeleton marker, at 1/50 dilution.
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Immunoprecipitation - Anti-TET3 antibody - C-terminal (ab153724)Immunoprecipitation of TET3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells.
IP Sample:
Lane 1: Transfected HEK-293T cell lysate.
Lane 2: Control with 2 µg of preimmune rabbit IgG
Lane 3: Immunoprecipitate of TET3-transfected HEK-293T cells using ab153724 at 2 µg.5% SDS-PAGE
Detection using ab153724 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab153724 は 4 報の論文で使用されています。
- Chen B et al. Regulation of paternal 5mC oxidation and H3K9me2 asymmetry by ERK1/2 in mouse zygotes. Cell Biosci 12:25 (2022). PubMed: 35255956
- Zhu M et al. TET3 governs malignant behaviors and unfavorable prognosis of esophageal squamous cell carcinoma by activating the PI3K/AKT/GSK3β/β-catenin pathway. Open Med (Wars) 17:1883-1895 (2022). PubMed: 36518116
- Guo Y et al. 5mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy. Front Cardiovasc Med 9:1053697 (2022). PubMed: 36620624
- Antunes C et al. Tet3 ablation in adult brain neurons increases anxiety-like behavior and regulates cognitive function in mice. Mol Psychiatry N/A:N/A (2020). PubMed: 32103150