Anti-TEAD4/TEF-3 抗体 [EPR26046-193]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TEAD4/TEF-3 with ab308621 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human skeletal muscle. The section was incubated with ab308621 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK-29 tissue labeling TEAD4/TEF-3 with ab308621 at 1/5000 (0.091 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) Wild-type HEK293T cell pellet and no staining on (B) TEAD4 knockout HEK-293T (ab257732) cell pellet. The section was incubated with ab308621 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling TEAD4/TEF-3 with ab308621 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human astrocytoma. The section was incubated with ab308621 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling TEAD4/TEF-3 with ab308621 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human breast cancer. The section was incubated with ab308621 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TEAD4/TEF-3 with ab308621 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human pancreas. The section was incubated with ab308621 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

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Immunoprecipitation - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

TEAD4/TEF-3 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with ab308621 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308621 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2 : ab308621 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab308621 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds.

All lanes:

Immunoprecipitation - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (ab308621) at 1/1000 dilution

All lanes:

293T (human embryonic kidney epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 32s

• WB

Supplier Data

Western blot - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, ab308621 was shown to bind specifically to TEAD4. Target of interest was observed at 34 kDa and 50 KDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in TEAD4 knockout cell line (lane 2). In Western Blot, we detect bands at ~50 KDa and ~34 KDa. Based on the immunogen sequence, this antibody is expected to detect isoforms Q15561-1 and Q15561-2, which correspond to full-length TEAD4 and TEAD4-S (PMID : 27291620). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds.

Lane 1:

Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate

Lane 2:

TEAD4 knockout HAP1 whole cell lysate

Observed band size: 34 kDa,50 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with human TEAD1, TEAD2 and TEAD3. Exposure time : 3 seconds.

Lane 1:

His-tagged human TEAD1 recombinant protein at 100 ng

Lane 2:

His-tagged human TEAD2 recombinant protein at 200 ng

Lane 3:

His-tagged human TEAD3 recombinant protein fragment

Lane 4:

His-tagged human TEAD4 recombinant protein fragment at 100 ng

Observed band size: 12 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-TEAD4/TEF-3 antibody [EPR26046-193] (AB308621)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western Blot, we detect bands at ~50 KDa and ~34 KDa. Basedon the immunogen sequence, this antibody is expected to detect isoforms Q15561-1 and Q15561-2, which correspond to full-length TEAD4 and TEAD4-S (PMID : 27291620). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds.

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Observed band size: 34 kDa,50 kDa

false

Exposure time: 15s