AB155244

Anti-TEAD4 抗体

Anti-TEAD4 antibody

JP Deleterious:医薬用外劇物

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(8 Publications)

Rabbit Polyclonal TEAD4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TEAD4 aa 100 to C-terminus.

別名を表示する

RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TEAD4 antibody (AB155244)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEAD4 antibody (AB155244)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TEAD4 with ab155244 at 1/200 in green and co-stained with Alpha-tubulin at 1 : 2000 in red.

Unknown

Western blot - Anti-TEAD4 antibody (AB155244)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TEAD4 antibody (ab155244) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 48 kDa

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Lab

Western blot - Anti-TEAD4 antibody (AB155244)

Blocking buffer and Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-TEAD4 antibody (ab155244) at 1/1000 dilution

Lane 1:

TEAD1 Human Recombinant Protein at 20 µg

Lane 2:

TEAD2 Human Recombinant Protein at 20 µg

Lane 3:

TEAD3 Human Recombinant Protein at 20 µg

Lane 4:

TEAD4 Human Recombinant Protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human TEAD4 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15561

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TEAD4 also known as TEF-3 or TEF is a transcription factor that plays a significant mechanical role in gene regulation. It belongs to the TEAD family of proteins. TEAD4 has an approximate mass of 48 kDa and is ubiquitously expressed in various tissues. It interacts with different cofactors to regulate gene expression and influences several cellular processes. The protein's expression is especially noted in cancerous tissues emphasizing its role in cellular growth and proliferation.

Biological function summary

TEAD4 is important for embryonic development and cell fate determination. It forms a complex with YAP (Yes-associated protein) to regulate transcriptional activities. The TEAD4-YAP complex binds to DNA driving the expression of genes involved in cell proliferation and survival. TEAD4 also coordinates with _tef_1 and several other transcription factors to regulate genes necessary for development and differentiation influencing processes such as muscle and neural tissue formation.

Pathways

TEAD4 is primarily involved in the Hippo signaling pathway known to regulate organ size by controlling cell proliferation and apoptosis. TEAD4 interacts with proteins like YAP and TAZ which play integral roles within this pathway. The Hippo pathway influences cell growth and TEAD4 as a mediator of this action contributes to the balance between cell proliferation and apoptosis. TEAD4's role extends to pathways involving cell cycle regulation and response to cellular stress.

TEAD4 exhibits significant relevance in cancer and cardiovascular diseases. Overexpression or dysregulation of TEAD4 often through its interaction with YAP is linked with tumorigenesis in breast and liver cancers. It promotes cancer cell proliferation migration and invasion. In the cardiovascular system TEAD4 impacts heart development and function with implications in congenital heart defects. Its interaction with proteins like _tef_1 also highlights its role in these diseases making it a potential therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.

See full target information TEAD4

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 44:1350-1373 PubMed39340215

2024

Yap methylation-induced FGL1 expression suppresses anti-tumor immunity and promotes tumor progression in KRAS-driven lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Jiang,Pengfei Ye,Ningning Sun,Weihua Zhu,Mei Yang,Manman Yu,Jingjing Yu,Hui Zhang,Zijie Gao,Ningjie Zhang,Shijie Guo,Yuru Ji,Siqi Li,Cuncun Zhang,Sainan Miao,Mengqi Chai,Wenmin Liu,Yue An,Jian Hong,Wei Wei,Shihao Zhang,Huan Qiu

Progress in orthodontics 25:29 PubMed39129034

2024

KAT6A/YAP/TEAD4 pathway modulates osteoclastogenesis by regulating the RANKL/OPG ratio on the compression side during orthodontic tooth movement.

Applications

Unspecified application

Species

Unspecified reactive species

Kuang Tan,Jiayi Wang,Xinyu Su,Yunfei Zheng,Weiran Li

iScience 27:109667 PubMed38966570

2024

upregulated by promotes ferroptosis via inhibiting axis in sepsis-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Lv,Jing Yu,Xingjia Qian,Jun Shu,Qiuhong Qian,Luhong Shen,Dongfang Shi,Zhengzheng Tao,Guiqin Fan,Bufeng Zhuang,Bing Lu

Journal of biomedical materials research. Part A 111:1781-1797 PubMed37494632

2023

Elastic modulus of hydrogel regulates osteogenic differentiation via liquid-liquid phase separation of YAP.

Applications

Unspecified application

Species

Unspecified reactive species

Kuang Tan,Qiaolin Yang,Yineng Han,Ziyao Zhuang,Yi Zhao,KunYao Guo,Anqi Tan,Yunfei Zheng,Weiran Li

Cell death discovery 8:59 PubMed35149670

2022

YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao Zhang

Stem cell research 56:102507 PubMed34454392

2021

Establishment of human induced trophoblast stem-like cells from term villous cytotrophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Bai,Chian-Yu Peng,Ivy Aneas,Noboru Sakabe,Daniela F Requena,Christine Billstrand,Marcelo Nobrega,Carole Ober,Mana Parast,John A Kessler

Molecular medicine reports 24: PubMed34396425

2021

HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Geng,Guangli Liu,Jiandong Hu,Jianchao Li,Dong Wang,Shenshan Zou,Xintao Xu

The Laryngoscope 125:E356-64 PubMed26308241

2015

The effectiveness of different neuroprotective agents in facial nerve injury: An experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Gokce Tanyeri,Onur Celik,Oytun Erbas,Fatih Oltulu,Ozlem Yilmaz Dilsiz

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TEAD4 抗体

Anti-TEAD4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TEAD4 antibody (AB155244)

Western blot - Anti-TEAD4 antibody (AB155244)

Western blot - Anti-TEAD4 antibody (AB155244)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise