Anti-TEAD4 抗体 [5H3] (ab58310)
![Western blot - Anti-TEAD4 antibody [5H3] (ab58310)](https://www.abcam.co.jp/ps/products/58/ab58310/Images/ab58310-536387-anti-tead4-antibody-5H3-western-blot-human.jpg "Western blot - Anti-TEAD4 antibody [5H3] (ab58310)")
Key features and details
- Mouse monoclonal [5H3] to TEAD4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-TEAD4 antibody [5H3]
TEAD4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5H3] to TEAD4 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 151-261 of Human TEAD4
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特記事項
This product was changed from ascites to tissue culture supernatant on 30th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
5H3 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58310の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
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| 特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. -
組織特異性
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. -
配列類似性
Contains 1 TEA DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7004 Human
- Omim: 601714 Human
- SwissProt: Q15561 Human
- Unigene: 94865 Human
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別名
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
see all
画像
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Western blot - Anti-TEAD4 antibody [5H3] (ab58310)All lanes : Anti-TEAD4 antibody [5H3] (ab58310) at 1/1000 dilution
Lane 1 : TEAD1 Human Recombinant Protein
Lane 2 : TEAD2 Human Recombinant Protein
Lane 3 : TEAD3 Human Recombinant Protein
Lane 4 : TEAD4 Human Recombinant Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 50-75 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-TEAD4 antibody [5H3] (ab58310)](https://www.abcam.co.jp/ps/products/58/ab58310/Images/ab58310-511408-anti-tead4-antibody-5h3-western-blot-wildtype-a549-tead4-knockout-a549-t47d.jpg)
Western blot - Anti-TEAD4 antibody [5H3] (ab58310)All lanes : Anti-TEAD4 antibody [5H3] (ab58310) at 1 µg
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TEAD4 CRISPR-Cas9 edited A549 cell lysate
Lane 3 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDaFalse colour image of Western blot: Anti-TEAD4 antibody [5H3] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab58310 was shown to bind specifically to TEAD4. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in TEAD4 CRISPR-Cas9 edited cell line ab277855. The band observed in the CRISPR-Cas9 edited lysate lane below 48 kDa is likely to represent a truncated form of TEAD4. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and TEAD4 CRISPR-Cas9 edited A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-TEAD4 antibody [5H3] (ab58310)](https://www.abcam.co.jp/ps/products/58/ab58310/Images/ab58310_1.jpg)
Western blot - Anti-TEAD4 antibody [5H3] (ab58310)TEAD4 antibody (ab58310) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (95)
ab58310 は 95 報の論文で使用されています。
- Wang YQ et al. TEAD4 is a master regulator of high-risk nasopharyngeal carcinoma. Sci Adv 9:eadd0960 (2023). PubMed: 36608137
- Ippolito F et al. Extracellular signal-Regulated Kinase 5 (ERK5) is required for the Yes-associated protein (YAP) co-transcriptional activity. Cell Death Dis 14:32 (2023). PubMed: 36650140
- Kim M et al. Super-enhancer-associated transcription factors collaboratively regulate trophoblast-active gene expression programs in human trophoblast stem cells. Nucleic Acids Res 51:3806-3819 (2023). PubMed: 36951126
- Karakis V et al. Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions. J Biol Chem 299:104650 (2023). PubMed: 36972789
- Zhao HC et al. CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis. iScience 26:106862 (2023). PubMed: 37275516
