Anti-TEA domain family member 2/ETF 抗体 [404C5a] (ab54374)
![Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)](https://www.abcam.co.jp/ps/products/54/ab54374/Images/ab54374-67217-anti-tea-domain-family-member-2-etf-antibody-404c5a-western-blot.gif "Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)")
Key features and details
- Mouse monoclonal [404C5a] to TEA domain family member 2/ETF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-TEA domain family member 2/ETF antibody [404C5a]
TEA domain family member 2/ETF 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [404C5a] to TEA domain family member 2/ETF -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human TEA domain family member 2/ETF.
Database link: Q15562 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
404C5a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. -
配列類似性
Contains 1 TEA DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- Embryonic TEA domain containing factor antibody
- Etdf antibody
- ETEF 1 antibody
see all
画像
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Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374) + immunizing recombinant protein
Predicted band size: 49 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab54374 は論文での使用が確認できていません。