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リコンビナントRabMAb

Anti-TdT 抗体 [EPR9732] (ab155082)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
  • Western blot - Anti-TdT antibody [EPR9732] (ab155082)
  • Anti-TdT antibody [EPR9732] (ab155082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9732] to TdT
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TdT antibody [EPR9732]
    TdT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9732] to TdT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human TdT.

  • ポジティブ･コントロール

    • Human fetal thymus and Jurkat cell lysates; Human thymoma and thymus tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9732

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab155082の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

  • 配列類似性

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Deoxynucleotidyltransferase terminal antibody
      • DNA nucleotidylexotransferase antibody
      • DNTT antibody
      • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
      • TDT antibody
      • TDT_HUMAN antibody
      • Terminal addition enzyme antibody
      • Terminal deoxynucleotidyltransferase antibody
      • Terminal deoxyribonucleotidyltransferase antibody
      • Terminal transferase antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

      Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling TdT with ab155082 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [EPR9732] (ab155082)

      Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling TdT with ab155082 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-TdT antibody [EPR9732] (ab155082)
      Western blot - Anti-TdT antibody [EPR9732] (ab155082)
      All lanes : Anti-TdT antibody [EPR9732] (ab155082) at 1/1000 dilution

      Lane 1 : Human fetal thymus cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 59 kDa

    • Anti-TdT antibody [EPR9732] (ab155082)
      Anti-TdT antibody [EPR9732] (ab155082)

    参考文献 (1)

    ab155082 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab155082 は 1 報の論文で使用されています。

    • Khoronenkova SV & Dianov GL ATM prevents DSB formation by coordinating SSB repair and cell cycle progression. Proc Natl Acad Sci U S A 112:3997-4002 (2015). PubMed: 25775545

    レビューと Q&A

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