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リコンビナントRabMAb

Anti-TdT 抗体 [EPR2975] (ab79395)

Reviews (1) Submit a question
Western blot - Anti-TdT antibody [EPR2975] (ab79395)
  • Anti-TdT antibody [EPR2975] (ab79395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2975] to TdT
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TdT antibody [EPR2975]
    TdT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2975] to TdT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human TdT aa 100-200. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Fetal thymus and Jurkat cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2975

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79395の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

  • 配列類似性

    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Deoxynucleotidyltransferase terminal antibody
      • DNA nucleotidylexotransferase antibody
      • DNTT antibody
      • Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
      • TDT antibody
      • TDT_HUMAN antibody
      • Terminal addition enzyme antibody
      • Terminal deoxynucleotidyltransferase antibody
      • Terminal deoxyribonucleotidyltransferase antibody
      • Terminal transferase antibody
      see all

    画像

    • Western blot - Anti-TdT antibody [EPR2975] (ab79395)
      Western blot - Anti-TdT antibody [EPR2975] (ab79395)
      All lanes : Anti-TdT antibody [EPR2975] (ab79395) at 1/2000 dilution

      Lane 1 : Fetal thymus cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Anti-TdT antibody [EPR2975] (ab79395)
      Anti-TdT antibody [EPR2975] (ab79395)

    参考文献 (0)

    ab79395 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab79395 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TdT antibody [EPR2975]

     Average
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Sample
    Mouse Cell (B cell (lymphocytes))
    Specification
    B cell (lymphocytes)
    Permeabilization
    Yes - 0.5% triton
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com