Anti-TDP1 抗体 (ab4166)
Key features and details
- Rabbit polyclonal to TDP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-TDP1 antibody
TDP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TDP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4166の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
Abcam recommends blocking with 1-3% Milk. |
| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa). Abcam recommends blocking with 1-3% Milk. |
ターゲット情報
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機能
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. -
組織特異性
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. -
関連疾患
Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy -
配列類似性
Belongs to the tyrosyl-DNA phosphodiesterase family. -
翻訳後修飾
Phosphorylated on serine and/or threonine residues, but not on tyrosine residues. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 55775 Human
- Entrez Gene: 104884 Mouse
- Entrez Gene: 314380 Rat
- Omim: 607198 Human
- SwissProt: Q9NUW8 Human
- SwissProt: Q8BJ37 Mouse
- SwissProt: Q4G056 Rat
- Unigene: 209945 Human
see all -
別名
- AI838772 antibody
- AW493413 antibody
- FLJ11090 antibody
see all
画像
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Western blot - Anti-TDP1 antibody (ab4166)Anti-TDP1 antibody (ab4166) at 1 µg/ml (blocked with 3% milk) + Jurkat nuclear extract lysate (ab14844) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Western blot - Anti-TDP1 antibody (ab4166)This image is courtesy of Keith Caldecott, University of SussexLane 1 : Molecular weight markers
Lanes 2-5 : Anti-TDP1 antibody (ab4166) at 1/1000 dilution
Lane 2 : 1 ng purified recombinant human TDP1
Lane 3 : 3 ng purified recombinant human TDP1
Lane 4 : 6 ng purified recombinant human TDP1
Lane 5 : 6 micrograms total protein from MCF7 human cell extract
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDaab4166 was incubated for 1 hr at room temp.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab4166 は 28 報の論文で使用されています。
- Bhattacharjee S et al. Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation. STAR Protoc 4:102218 (2023). PubMed: 37058403
- Marini V et al. MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links. BMC Biol 21:110 (2023). PubMed: 37194054
- Meroni A et al. NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors. Sci Adv 8:eabq0648 (2022). PubMed: 36490343
- Leung E et al. Validating TDP1 as an Inhibition Target for the Development of Chemosensitizers for Camptothecin-Based Chemotherapy Drugs. Oncol Ther 9:541-556 (2021). PubMed: 34159519
- Álvarez-Quilón A et al. Endogenous DNA 3' Blocks Are Vulnerabilities for BRCA1 and BRCA2 Deficiency and Are Reversed by the APE2 Nuclease. Mol Cell 78:1152-1165.e8 (2020). PubMed: 32516598