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リコンビナントRabMAb

Anti-TDG 抗体 [EPR8774] (ab154192)

Reviews (1) Submit a question References (4)
Western blot - Anti-TDG antibody [EPR8774] (ab154192)
  • Western blot - Anti-TDG antibody [EPR8774] (ab154192)
  • Flow Cytometry (Intracellular) - Anti-TDG antibody [EPR8774] (ab154192)
  • Anti-TDG antibody [EPR8774] (ab154192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8774] to TDG
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TDG antibody [EPR8774]
    TDG 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8774] to TDG

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human TDG.
    Database link: Q13569

  • ポジティブ･コントロール

    • Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR8774

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154192の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

  • 配列類似性

    Belongs to the TDG/mug DNA glycosylase family.

  • 翻訳後修飾

    Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C JUN leucine zipper interactive protein antibody
    • C JUN leucine zipper interactive protein antibody
    • C-JUN leucine zipper interactive protein JZA-3 antibody
    • E130317C12Rik antibody
    • EC 3.2.2.29 antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch specific thymine DNA glycosylase antibody
    • G/T mismatch-specific thymine DNA glycosylase antibody
    • JZA 3 antibody
    • Jza1 antibody
    • T:G mismatch thymine glycosylase antibody
    • Tdg antibody
    • TDG_HUMAN antibody
    • Thymine DNA glycosylase antibody
    • Thymine-DNA glycosylase antibody
    see all

画像

  • Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 46 kDa
    Observed band size: 55-60 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/1000 dilution

    Lane 1 : Fetal lung cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : Fetal brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Flow Cytometry (Intracellular) - Anti-TDG antibody [EPR8774] (ab154192)
    Flow Cytometry (Intracellular) - Anti-TDG antibody [EPR8774] (ab154192)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TDG with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green). 

     

     

  • Anti-TDG antibody [EPR8774] (ab154192)
    Anti-TDG antibody [EPR8774] (ab154192)

参考文献 (4)

ab154192 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154192 は 4 報の論文で使用されています。

  • Fan BF  et al. Deficiency of Tet3 in nucleus accumbens enhances fear generalization and anxiety-like behaviors in mice. Brain Pathol 32:e13080 (2022). PubMed: 35612904
  • Nanan KK  et al. TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide. iScience 19:326-339 (2019). PubMed: 31404833
  • Wu X  et al. Porcine Circovirus Type 2 Rep Enhances IL-10 Production in Macrophages via Activation of p38-MAPK Pathway. Viruses 11:N/A (2019). PubMed: 31835539
  • Neri F  et al. Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics. Cell Rep N/A:N/A (2015). PubMed: 25660018

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-TDG antibody [EPR8774]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (primary human fibroblasts)
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine)
Loading amount
40 µg
Treatment
control or anti-TDG siRNA
Specification
primary human fibroblasts
Blocking step
Odyssey-Blocking buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Enni Markkanen

Verified customer

投稿 Feb 09 2016

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