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AB314167

Anti-TCTP 抗体 [10A9]

Anti-TCTP antibody [10A9]

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Rabbit Monoclonal TCTP antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Translationally-controlled tumor protein.

別名を表示する

Translationally-controlled tumor protein, TCTP, Fortilin, Histamine-releasing factor, p23, HRF, TPT1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCTP antibody [10A9] (AB314167)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCTP antibody [10A9] (AB314167)

Immunohistochemical analysis of ab314167 diluted at 1 : 100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [10A9] (AB314167)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [10A9] (AB314167)

Immunofluorescence staining of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells with ab314167 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Immunoprecipitation - Anti-TCTP antibody [10A9] (AB314167)
  • IP

Supplier Data

Immunoprecipitation - Anti-TCTP antibody [10A9] (AB314167)

Immunoprecipitating TCTP in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 1 : Rabbit control IgG instead of ab314167 in HeLa whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2 : ab314167 (2µg)+ HeLa whole cell lysate (500µg) Lane 3 : HeLa whole cell lysate (10µg)

All lanes:

Immunoprecipitation - Anti-TCTP antibody [10A9] (ab314167) at 2 µg

Predicted band size: 20 kDa

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Western blot - Anti-TCTP antibody [10A9] (AB314167)
  • WB

Supplier Data

Western blot - Anti-TCTP antibody [10A9] (AB314167)

All lanes:

Western blot - Anti-TCTP antibody [10A9] (ab314167) at 1/2000 dilution

Lane 1:

HeLa whole cell lysate

Lane 2:

Jurkat whole cell lysate

Lane 3:

MCF7 whole cell lysate

Lane 4:

RAW264.7 whole cell lysate

Lane 5:

HepG2 whole cell lysate

Lane 6:

PC-3 whole cell lysate

Lane 7:

Raji whole cell lysate

Lane 8:

HEK293 whole cell lysate

Lane 9:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Observed band size: 25 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

10A9

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

IHC-P, ICC/IF, WB, IP

applications

免疫原

Synthetic Peptide within Human Translationally-controlled tumor protein.

P13693

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/1000", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TCTP also known as Translationally Controlled Tumor Protein or histamine-releasing factor (HRF) is a multifunctional protein with a molecular mass of approximately 19 kDa. It is found in a variety of tissues and cell types including cytoplasm and extracellular environments. The protein engages in cellular processes such as cell growth development and apoptosis. TCTP regulates microtubule stability cell cycle progression and protein synthesis reflecting its broad mechanical roles within cells.
Biological function summary

In both animal and human cells TCTP acts as a prosurvival factor by inhibiting apoptosis. It binds with proteins like Bcl-2 and Bax playing a role in the regulation of cell death. Furthermore TCTP appears to mediate immune responses through its histamine-releasing factor activity. It interacts within protein complexes that manage cytoskeletal dynamics and stress responses contributing to cellular adaptability.

Pathways

The protein interacts in critical pathways such as the MAPK signaling pathway and the apoptosis pathway. In the MAPK pathway TCTP modulates cell growth and proliferation by interacting with proteins like ERK1/2. In the apoptosis pathway TCTP's interaction with Bcl-xL and Bax highlights its role in the fine-tuning of cell survival and death processes. These pathways highlight its participation in essential cellular functions and disease progression.

TCTP has implications in cancer and allergy. Altered expression and function of TCTP have been linked to tumor growth and resistance to cancer therapies often regulated through complex interactions with proteins like p53. In allergies its role in histamine release positions it as a mediator in allergic asthma and other hypersensitivity reactions. These associations emphasize the clinical relevance of TCTP and its potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in calcium binding and microtubule stabilization (PubMed : 12167714, PubMed : 15162379, PubMed : 15958728). Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein (PubMed : 18325344).
See full target information Translationally-controlled tumor protein

追加のターゲット

Tpt1
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組合せ製品

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Primary Antibodies

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  • 0
  • 0
Primary Antibodies

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Primary Antibodies

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