Anti-TCP1 theta 抗体 (ab176691)
Key features and details
- Rabbit polyclonal to TCP1 theta
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TCP1 theta antibody
TCP1 theta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TCP1 theta -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Cow, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide within Human TCP1 theta aa 498-548. The exact sequence is proprietary.
Sequence:LDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQN D
Database link: P50990 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat or Mouse NIH3T3 lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH: 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176691の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 60 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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関連性
TCP1 theta is the theta polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double ring. TCP1 theta is a molecular chaperone that assists the folding of proteins upon ATP hydrolysis. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. -
細胞内局在
Cytoplasm. Cytoplasm; cytoskeleton; centrosome -
参照データベース
- Entrez Gene: 418486 Chicken
- Entrez Gene: 281047 Cow
- Entrez Gene: 10694 Human
- Entrez Gene: 12469 Mouse
- SwissProt: Q6EE31 Chicken
- SwissProt: Q3ZCI9 Cow
- SwissProt: P50990 Human
- SwissProt: P42932 Mouse
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別名
- C21orf112 antibody
- CCT 8 antibody
- CCT theta antibody
see all
画像
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Immunoprecipitation - Anti-TCP1 theta antibody (ab176691)
Detection of Human TCP1 theta by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176691 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated TCP1 theta, ab176691 was used at 0.04 µg/ml.
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Western blot - Anti-TCP1 theta antibody (ab176691)All lanes : Anti-TCP1 theta antibody (ab176691) at 0.04 µg/ml
Lane 1 : HeLa lysate at 50 µg
Lane 2 : HeLa lysate at 15 µg
Lane 3 : 293T lysate at 50 µg
Lane 4 : Jurkat lysate at 50 µg
Lane 5 : Mouse NIH3T3 lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab176691 は論文での使用が確認できていません。
