Anti-TCOF1 抗体 (ab65212)
Key features and details
- Rabbit polyclonal to TCOF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TCOF1 antibody
TCOF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TCOF1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TCOF1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65212の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
May be involved in nucleolar-cytoplasmic transport. May play a fundamental role in early embryonic development, particularly in development of the craniofacial complex. -
関連疾患
Defects in TCOF1 are the cause of Treacher Collins syndrome type 1 (TCS1) [MIM:154500]. It is a form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. -
配列類似性
Contains 1 LisH domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 6949 Human
- Omim: 154500 Human
- SwissProt: Q13428 Human
- Unigene: 519672 Human
- Unigene: 605019 Human
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別名
- Mandibulofacial dysostosis antibody
- MFD1 antibody
- Nucleolar trafficking phosphoprotein antibody
see all
画像
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Western blot - Anti-TCOF1 antibody (ab65212)All lanes : Anti-TCOF1 antibody (ab65212) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells, plus immunising peptide
Predicted band size: 152 kDa
Observed band size: 152 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCOF1 antibody (ab65212)Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab65212 at a 1:50 dilution.
Left image untreated.Right image treated with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab65212 は 5 報の論文で使用されています。
- Wang F et al. Genetic mutations in ribosomal biogenesis gene TCOF1 identified in human neural tube defects. Mol Genet Genomic Med 11:e2150 (2023). PubMed: 36808708
- Dash S et al. rRNA transcription is integral to phase separation and maintenance of nucleolar structure. PLoS Genet 19:e1010854 (2023). PubMed: 37639467
- Franek M et al. Nucleolar Reorganization Upon Site-Specific Double-Strand Break Induction. J Histochem Cytochem 64:669-686 (2016). PubMed: 27680669
- Stixová L et al. HP1ß-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. Epigenetics Chromatin 7:39 (2014). ICC/IF ; Mouse . PubMed: 25587355
- Zentner GE et al. CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis. Hum Mol Genet 19:3491-501 (2010). WB, ChIP ; Mouse . PubMed: 20591827