Anti-TCF3 / E2A 抗体 (ab69999)
Key features and details
- Rabbit polyclonal to TCF3 / E2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TCF3 / E2A antibody
TCF3 / E2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TCF3 / E2A -
由来種
Rabbit -
特異性
Detects endogenous levels of total TCF3/E2A protein. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
-
免疫原
Synthesized peptide derived from the C terminus of human TCF3/E2A
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69999の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
|
| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
-
機能
Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. -
関連疾患
Note=Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻訳後修飾
Phosphorylated following NGF stimulation. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 6929 Human
- Entrez Gene: 171046 Rat
- Omim: 147141 Human
- SwissProt: P15923 Human
- SwissProt: P21677 Rat
- Unigene: 371282 Human
- Unigene: 10290 Rat
-
別名
- AGM8 antibody
- bHLHb21 antibody
- Class B basic helix-loop-helix protein 21 antibody
see all
画像
-
Western blot - Anti-TCF3 / E2A antibody (ab69999)All lanes : Anti-TCF3 / E2A antibody (ab69999) at 1/500 dilution
Lane 1 : Extracts from 293 cells, minus immunising peptide
Lane 2 : Extracts from 293 cells, plus immunising peptide 5µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF3 / E2A antibody (ab69999)ab69999, at a 1/50 dilution, staining human TCF3/E2A in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab69999 は 6 報の論文で使用されています。
- Yang F et al. p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation. Mol Cancer 20:123 (2021). PubMed: 34579723
- Song Y et al. Interleukin-6 (IL-6) Activates the NOTCH1 Signaling Pathway Through E-Proteins in Endometriotic Lesions. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32119078
- Ahmed NS et al. Epidermal E-Cadherin Dependent ß-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling. Mol Ther 25:2502-2512 (2017). PubMed: 28803863
- Loriot C et al. Deciphering the molecular basis of invasiveness in Sdhb-deficient cells. Oncotarget 6:32955-65 (2015). WB . PubMed: 26460615
- Li C et al. Hypomethylation-associated up-regulation of TCF3 expression and recurrence in stage II and III colorectal cancer. PLoS One 9:e112005 (2014). IHC ; Human . PubMed: 25375219
- Lee K et al. Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. EMBO J 30:2662-74 (2011). IF, WB ; Mouse . PubMed: 21610693