Anti-TCF12 抗体 (ab70746)
Key features and details
- Rabbit polyclonal to TCF12
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TCF12 antibody
TCF12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TCF12 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 1 and 50 of human TCF12 (NP_996919.1).
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ポジティブ･コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70746の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 73 kDa).
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| 特記事項 |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 73 kDa). |
ターゲット情報
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機能
Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. -
組織特異性
Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 509039 Cow
- Entrez Gene: 6938 Human
- Entrez Gene: 21406 Mouse
- Entrez Gene: 25720 Rat
- Omim: 600480 Human
- SwissProt: Q99081 Human
- SwissProt: Q61286 Mouse
- SwissProt: P51514 Rat
see all -
別名
- bHLHb20 antibody
- Class B basic helix-loop-helix protein 20 antibody
- DNA binding protein HTF4 antibody
see all
画像
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Western blot - Anti-TCF12 antibody (ab70746)All lanes : Anti-TCF12 antibody (ab70746) at 1/10000 dilution
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 120 kDa, 190 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCF12 antibody (ab70746)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TCF12 with ab70746 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-TCF12 antibody (ab70746)1mg whole cell lysate from HeLa cells was immunprecipitated with ab70746 at 3µg/mg lysate. For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab707345 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab70746 は 5 報の論文で使用されています。
- Zheng K et al. CircRNA PIP5K1A promotes the progression of glioma through upregulation of the TCF12/PI3K/AKT pathway by sponging miR-515-5p. Cancer Cell Int 21:27 (2021). PubMed: 33413401
- Zhu G et al. P53/miR-154 Pathway Regulates the Epithelial-Mesenchymal Transition in Glioblastoma Multiforme Cells by Targeting TCF12. Neuropsychiatr Dis Treat 17:681-693 (2021). PubMed: 33664574
- Lu F et al. LncRNA MIAT promotes the proliferation, migration, and invasion of melanoma cells through recruiting TCF12 and activating NFAT5. Am J Transl Res 13:12588-12600 (2021). PubMed: 34956475
- Song Y et al. Interleukin-6 (IL-6) Activates the NOTCH1 Signaling Pathway Through E-Proteins in Endometriotic Lesions. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32119078
- Wang M et al. The mechanism of Chinese herbal formula HQT in the treatment of rheumatoid arthritis is related to its regulation of lncRNA uc.477 and miR-19b. J Leukoc Biol 108:519-529 (2020). PubMed: 32696503