AB225627

Anti-TCF-25 抗体

Name: Anti-TCF-25 抗体 (ab225627) | Abcam
Brand: Abcam Limited
SKU: AB225627
Price: 74000 JPY
Availability: InStock

Anti-TCF-25 antibody

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(1 Publication)

Rabbit Polyclonal TCF-25 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TCF25 aa 1-50.

別名を表示する

KIAA1049, NULP1, FKSG26, TCF25, Ribosome quality control complex subunit TCF25, Nuclear localized protein 1, Transcription factor 25, TCF-25

2 Images

Supplier Data

Immunoprecipitation - Anti-TCF-25 antibody (AB225627)

TCF-25 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225627 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225627 at 0.4 μg/ml.

Lane 1 : ab225627 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TCF-25 antibody (ab225627)

Predicted band size: 77 kDa

false

Supplier Data

Western blot - Anti-TCF-25 antibody (AB225627)

All lanes:

Western blot - Anti-TCF-25 antibody (ab225627) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human TCF25 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ70

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab225627 was affinity purified using an epitope specific to TCF-25 immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TCF-25 also known as Transcription Factor 25 plays an important role in gene expression by regulating transcription processes. Its protein mass is approximately 25 kDa. TCF-25 is expressed broadly across several tissues with particular prominence in the brain and heart. This expression pattern suggests its involvement in critical cellular processes in these organ systems.

Biological function summary

TCF-25 modulates expression of genes involved in development and differentiation. It functions as a part of a complex with other transcription factors to regulate target genes. This complex interaction enhances its ability to control biological processes maintaining cellular homeostasis and supporting the progression of various developmental stages.

Pathways

Studies show TCF-25 plays an influential role in both Wnt and Notch signaling pathways. These pathways are important for cell fate determination and tissue development. Within these pathways TCF-25 interacts with proteins such as beta-catenin in the Wnt signaling pathway and works alongside Notch receptors in the Notch signaling pathway illustrating its integration into these significant cellular signaling networks.

Research finds TCF-25 linked to cardiovascular diseases and neuronal development disorders. Abnormalities in TCF-25 expression or function contribute to pathogenesis in conditions like congenital heart defects where its partnership with proteins like GATA4 and NKX2-5 is essential. Additionally misregulation of TCF-25 can impact neural processes potentially leading to neurological disorders through its interactions with other transcription factors.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation (PubMed : 30244831). In the RQC complex, required to promote formation of 'Lys-48'-linked polyubiquitin chains during ubiquitination of incompletely synthesized proteins by LTN1 (PubMed : 30244831). Also acts as a transcriptional repressor : represses transcription of SRF in vitro and so may play a role in heart development (PubMed : 16574069). May play a role in cell death control (By similarity).

See full target information TCF25

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 30:1007-1017 PubMed36973424

2023

E2F1-mediated KDM4A-AS1 up-regulation promotes EMT of hepatocellular carcinoma cells by recruiting ILF3 to stabilize AURKA mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Ming Shen,Di Zhang,Ping Xiao,Bin Qu,Yi-Fan Sun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCF-25 抗体

Anti-TCF-25 antibody

Immunoprecipitation - Anti-TCF-25 antibody (AB225627)

Western blot - Anti-TCF-25 antibody (AB225627)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise