Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-TCEB2/Elongin-B 抗体 [EPR10441(B)] (ab154854)

Submit a review Submit a question References (2)
Flow Cytometry (Intracellular) - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
  • Western blot - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
  • Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10441(B)] to TCEB2/Elongin-B
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-TCEB2/Elongin-B antibody [EPR10441(B)]
    TCEB2/Elongin-B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10441(B)] to TCEB2/Elongin-B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human TCEB2/Elongin-B.
    Database link: Q15370

  • ポジティブ･コントロール

    • WB: Jurkat, A431, K562 cell lysates. Flow cyto (intra): Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154854の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
    The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 1 ubiquitin-like domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EloB antibody
      • ELOB_HUMAN antibody
      • Elongin 18 kDa subunit antibody
      • Elongin B antibody
      • Elongin-B antibody
      • RNA polymerase II transcription factor SIII p18 subunit antibody
      • RNA polymerase II transcription factor SIII subunit B antibody
      • SIII antibody
      • SIII p18 antibody
      • TCEB 2 antibody
      • Tceb2 antibody
      • Transcription elongation factor B (SIII) polypeptide 2 antibody
      • Transcription elongation factor B polypeptide 2 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
      Flow Cytometry (Intracellular) - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)

      Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling TCEB2/Elongin-B with purified ab154854 at 1/20 dilution (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as a secondary antibody at 1/2000 dilution. Isotype control -Rabbit monoclonal IgG (Black). Unlableled control -Cell without incubation with primary antibody and secondary antibody (Blue).

    • Western blot - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
      Western blot - Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
      All lanes : Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854) at 1/1000 dilution

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
      Lane 3 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 13 kDa
      Observed band size: 15 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concnetration: 5% NFDM/TBST.

      ab181602, anti GAPDH antibody was used as loading control.

    • Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)
      Anti-TCEB2/Elongin-B antibody [EPR10441(B)] (ab154854)

    参考文献 (2)

    ab154854 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154854 は 2 報の論文で使用されています。

    • Li K  et al. SOCS2 regulation of growth hormone signaling requires a canonical interaction with phosphotyrosine. Biosci Rep 42:N/A (2022). PubMed: 36398696
    • Hüttenhain R  et al. ARIH2 Is a Vif-Dependent Regulator of CUL5-Mediated APOBEC3G Degradation in HIV Infection. Cell Host Microbe 26:86-99.e7 (2019). PubMed: 31253590

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab154854.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com