Anti-TCEB2/Elongin-B 抗体 [EPR10440(B)] (ab151743)
![Western blot - Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)](https://www.abcam.co.jp/ps/products/151/ab151743/Images/ab151743-158843-anti-tceb2-elongin-b-antibody-epr10440b-western-blot.jpg "Western blot - Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10440(B)] to TCEB2/Elongin-B
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TCEB2/Elongin-B antibody [EPR10440(B)]
TCEB2/Elongin-B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10440(B)] to TCEB2/Elongin-B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TCEB2/Elongin-B aa 1-100. The exact sequence is proprietary.
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ポジティブ･コントロール
- Jurkat, HeLa, K562 and A431 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10440(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151743の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
ターゲット情報
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機能
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 ubiquitin-like domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6923 Human
- Omim: 600787 Human
- SwissProt: Q15370 Human
- Unigene: 172772 Human
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別名
- EloB antibody
- ELOB_HUMAN antibody
- Elongin 18 kDa subunit antibody
see all
画像
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Western blot - Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)All lanes : Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa -
![Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)](https://www.abcam.co.jp/ps/products/151/ab151743/Images/ab151743-2-benefits-of-recombinant-antibodies.png)
Anti-TCEB2/Elongin-B antibody [EPR10440(B)] (ab151743)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab151743 は 1 報の論文で使用されています。
- Zhang W et al. Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the receptor protein of CRL2Prame complex. Cell Death Differ 28:1926-1940 (2021). PubMed: 33504946