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リコンビナントRabMAb

Anti-TCEA1 抗体 [EPR15036] (ab184158)

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Western blot - Anti-TCEA1 antibody [EPR15036] (ab184158)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036] (ab184158)
  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036] (ab184158)
  • Anti-TCEA1 antibody [EPR15036] (ab184158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15036] to TCEA1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TCEA1 antibody [EPR15036]
    TCEA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15036] to TCEA1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, HEK293, K562 cell lysates; HUVEC cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184158の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/250.
IP
1/60 - 1/80.
特記事項
WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/250.
IP
1/60 - 1/80.

ターゲット情報

  • 機能

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

  • 関連疾患

    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

  • 配列類似性

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

画像

  • Western blot - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Western blot - Anti-TCEA1 antibody [EPR15036] (ab184158)
    All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR15036] (ab184158)

    Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells, staining TCEA1 (red) with ab184158 at 1/250 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR15036] (ab184158)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR15036] (ab184158)

    Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate, labeling TCEA1 immunoprecipitated using ab184158 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-TCEA1 antibody [EPR15036] (ab184158)
    Anti-TCEA1 antibody [EPR15036] (ab184158)

参考文献 (0)

ab184158 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184158 は論文での使用が確認できていません。

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