Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-TCEA1 抗体 [EPR14820(B)] (ab184181)

Submit a review Submit a question
Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
  • Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
  • Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14820(B)] to TCEA1
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TCEA1 antibody [EPR14820(B)]
    TCEA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14820(B)] to TCEA1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, HeLa, and SP2/0 cell lysate. Human lung adenocarcinoma and colon tissue. HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184181の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/250.
IP
1/40 - 1/60.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ICC/IF
1/250.
IP
1/40 - 1/60.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

  • 関連疾患

    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

  • 配列類似性

    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

画像

  • Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Western blot - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    All lanes : Anti-TCEA1 antibody [EPR14820(B)] (ab184181) at 1/10000 dilution

    Lane 1 : K652 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SP2/0 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunoprecipitation - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab184181 at 1/50 dilution.  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated used as secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunohistochemistry analysis of Human lung adenocarcinoma tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunohistochemistry analysis of Human colon tissue labeling TCEA1 with ab184181 at 1/100 dilution. Secondary antibody HRP conjugated Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Immunocytochemistry/ Immunofluorescence - Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

    Immunocytochemistry analysis of a HeLa cells labeling TCEA1 with ab184181 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Dapi counterstain shown in blue.

  • Anti-TCEA1 antibody [EPR14820(B)] (ab184181)
    Anti-TCEA1 antibody [EPR14820(B)] (ab184181)

参考文献 (0)

ab184181 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184181 は論文での使用が確認できていません。

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab184181.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com