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リコンビナントRabMAb

Anti-TC10 抗体 [Y304] (ab32079)

Reviews (1)Q&A (3)References (6)
Western blot - Anti-TC10 antibody [Y304] (ab32079)
  • Immunocytochemistry/ Immunofluorescence - Anti-TC10 antibody [Y304] (ab32079)
  • Anti-TC10 antibody [Y304] (ab32079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y304] to TC10
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TC10 antibody [Y304]
    TC10 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y304] to TC10

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293 cell lysate, HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32079の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF
1/250 - 1/500.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF
1/250 - 1/500.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.

  • 配列類似性

    Belongs to the small GTPase superfamily. Rho family.

  • 翻訳後修飾

    May be post-translationally modified by both palmitoylation and polyisoprenylation.

  • 細胞内局在

    Cytoplasm. Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ARHQ antibody
      • Ras homolog gene family member Q antibody
      • RAS like family 7 member A antibody
      • Ras like protein family member 7A antibody
      • Ras like protein TC10 antibody
      • Ras related GTP binding protein TC10 antibody
      • Ras-like protein family member 7A antibody
      • Ras-like protein TC10 antibody
      • RASL7A antibody
      • Rho related GTP binding protein RhoQ antibody
      • Rho-related GTP-binding protein RhoQ antibody
      • Rhoq antibody
      • RHOQ_HUMAN antibody
      • TC10A antibody
      see all

    画像

    • Western blot - Anti-TC10 antibody [Y304] (ab32079)
      Western blot - Anti-TC10 antibody [Y304] (ab32079)
      Anti-TC10 antibody [Y304] (ab32079) at 1/1000 dilution + 293 cell lysate

      Predicted band size: 23 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-TC10 antibody [Y304] (ab32079)
      Immunocytochemistry/ Immunofluorescence - Anti-TC10 antibody [Y304] (ab32079)
      Immunofluorescent analysis of TC10 expression in HeLa cells using 1/250 ab 32079.
    • Anti-TC10 antibody [Y304] (ab32079)
      Anti-TC10 antibody [Y304] (ab32079)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (6)

    ab32079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32079 は 6 報の論文で使用されています。

    • Koch D  et al. Obscurin Rho GEF domains are phosphorylated by MST-family kinases but do not exhibit nucleotide exchange factor activity towards Rho GTPases in vitro. PLoS One 18:e0284453 (2023). PubMed: 37079638
    • Liang J  et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). PubMed: 29670091
    • Fujita A  et al. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. PLoS One 8:e79689 (2013). ICC/IF . PubMed: 24223996
    • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . PubMed: 20733052
    • Fausther-Bovendo H  et al. HIV gp41 engages gC1qR on CD4+ T cells to induce the expression of an NK ligand through the PIP3/H2O2 pathway. PLoS Pathog 6:e1000975 (2010). WB ; Human . PubMed: 20617170
    • Yasuda S  et al. An essential role of Cdc42-like GTPases in mitosis of HeLa cells. FEBS Lett 580:3375-80 (2006). PubMed: 16716304

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-TC10 antibody [T304]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (embryonic dorsal root ganglia)
    Loading amount
    30 µg
    Specification
    embryonic dorsal root ganglia
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 03 2010

    Question

    Thank you very much for your support.
    The customer would like to get a credit note as suggested, your good attitude will no doubt encourage him to work with us in the future as well.

    Thanks again.
    Regards,

    Read More
    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: ####


    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Question

    Here is the customer reply:

    'The endogenous control sample was HEK293T cell lysate created using pEF plasmid. Marking with anti TC10 showed no band, marking with anti-flag produced a band.'

    Thank you for the kind support and a good week to you.
    Regards,

    Read More
    Answer

    Thank you fo ryour reply and for confirming these details.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I would still suggest it would be beneficial to considertrying the experiment again with a suitable endogenous positive control, such as HeLa or293 cells, as previously discussed. So you are detecting the native protein and not a recombinant protein.

    However, as an exception in this case, I am pleased to offer you a free of charge replacement or credit note in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Here is the lysis buffer ingredients list:
    WCE- 50ml
    Initial Con.
    Volume
    Hepes 1M
    1.25ml
    NaCl 5M
    3ml
    MgCl2 1M
    75µl
    EDTA 0.5M



    20µl
    Triton 10%
    500µl
    DDW45ml

    Read More
    Answer

    Thank you for taking the time to provide these details. Theinformation you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this case, I would like to offer some suggestions to help optimise the results. I would also appreciate if you can confirm some further details:

    1. There are inherent difficulties with antibody detection of recombinant proteins that I can recommend considering, particularly if they are tagged. This may be a biological artifact rather than a problem with the antibody.Could you confirm that the protein transfected is full length and includes the immunogen sequence? Also, if the recombinant TC10 is tagged, it is possible that the tag is preventing access to the antibody. Could you confirm at which region of the protein the tag has been placed?

    2. Could you confirm the species from which the recombinant TC10 gene was taken?

    3. Could you confirm how the stability of transfection has been assessed?

    4. I can recommend that due to the points described above, it is important to use an endogenous positive control sample. For example, this antibody has been used successfully on293 cell lysate.

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results with an endogenous positive control, please do not hesitate to contact me again with the further requested details.

    Read More

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