Anti-TC10 抗体 (ab168645)
Key features and details
- Mouse polyclonal to TC10
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TC10 antibody
TC10 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to TC10 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant full length protein within Human TC10 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P17081 -
ポジティブ･コントロール
- TC10 transfected 293T cell lysate. HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168645の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
|
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia. -
配列類似性
Belongs to the small GTPase superfamily. Rho family. -
翻訳後修飾
May be post-translationally modified by both palmitoylation and polyisoprenylation. -
細胞内局在
Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23433 Human
- Entrez Gene: 104215 Mouse
- Entrez Gene: 85428 Rat
- Omim: 605857 Human
- SwissProt: P17081 Human
- SwissProt: Q8R527 Mouse
- SwissProt: Q9JJL4 Rat
- Unigene: 709193 Human
see all -
別名
- ARHQ antibody
- Ras homolog gene family member Q antibody
- RAS like family 7 member A antibody
see all
画像
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Western blot - Anti-TC10 antibody (ab168645)All lanes : Anti-TC10 antibody (ab168645) at 1 µg/ml
Lane 1 : TC10 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TC10 antibody (ab168645)Immunofluorescence analysis of HeLa cells, labeling TC10 with ab168645 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab168645 は 1 報の論文で使用されています。
- Mayer S et al. Collybistin activation by GTP-TC10 enhances postsynaptic gephyrin clustering and hippocampal GABAergic neurotransmission. Proc Natl Acad Sci U S A 110:20795-800 (2013). PubMed: 24297911
