Anti-Tbx6 抗体 (ab38883)
Key features and details
- Rabbit polyclonal to Tbx6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tbx6 antibody
Tbx6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tbx6 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse Tbx6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab26243) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38883の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa). |
ターゲット情報
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機能
Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation. -
組織特異性
Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus. -
配列類似性
Contains 1 T-box DNA-binding domain. -
発生段階
Expressed during gastrulation and during a second phase in some adult tissues. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6911 Human
- Entrez Gene: 21389 Mouse
- Omim: 602427 Human
- SwissProt: O95947 Human
- SwissProt: P70327 Mouse
- Unigene: 198301 Human
- Unigene: 727 Mouse
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別名
- DFNB 67 antibody
- DFNB67 antibody
- SCDO5 antibody
see all
画像
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Western blot - Anti-Tbx6 antibody (ab38883)All lanes : Anti-Tbx6 antibody (ab38883) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab38883 は 6 報の論文で使用されています。
- Budjan C et al. Paraxial mesoderm organoids model development of human somites. Elife 11:N/A (2022). PubMed: 35088712
- Sanaki-Matsumiya M et al. Periodic formation of epithelial somites from human pluripotent stem cells. Nat Commun 13:2325 (2022). PubMed: 35484123
- Bredenkamp N et al. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency. Stem Cell Reports 13:1083-1098 (2019). PubMed: 31708477
- Liu C et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). PubMed: 30008328
- Chal J et al. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. Nat Protoc 11:1833-50 (2016). PubMed: 27583644
- Gouti M et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol 12:e1001937 (2014). ICC/IF ; Mouse . PubMed: 25157815