AB38883

Anti-Tbx6 抗体

Name: Anti-Tbx6 抗体 (ab38883) | Abcam
Brand: Abcam Limited
SKU: AB38883
Price: 59000 JPY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Tbx6 antibody

(3 Reviews)

(8 Publications)

Rabbit Polyclonal TBX6 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 8 publications.

別名を表示する

T-box transcription factor TBX6, T-box protein 6

1 Images

Project

Western blot - Anti-Tbx6 antibody (AB38883)

All lanes:

Western blot - Anti-Tbx6 antibody (ab38883) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

Lung (Mouse) Tissue Lysate at 10 µg

Lane 3:

Human testis tissue lysate - total protein (ab30257) at 10 µg

Lane 4:

Lung (Human) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 47 kDa

Observed band size: 43 kDa,50 kDa

false

Exposure time: 20min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tbx6 also known as T-box 6 is a transcription factor with a molecular mass of approximately 51 kDa. It belongs to the T-box family which is characterized by a conserved DNA-binding domain. Tbx6 is expressed in the paraxial mesoderm an area of embryonic tissue that eventually forms somites the precursors of skeletal muscle vertebrae and dermis. The expression of Tbx6 is highly regulated during developmental stages indicating its role in embryogenesis and tissue specification.

Biological function summary

Transcription factor Tbx6 plays a significant role in mesoderm specification and somite segmentation. It functions by binding to specific DNA sequences to regulate the expression of target genes essential for somite formation. Tbx6 does not form part of any known protein complex but it interacts with other transcription factors to influence mesoderm differentiation. The precise regulation of Tbx6 activity is important for proper embryonic development ensuring that the cells undertake their correct differentiation pathways.

Pathways

Tbx6 functions in pathways critical for embryonic development including the Wnt and Notch signaling pathways. The Wnt signaling pathway regulates cell fate determination while the Notch signaling controls segmentation during somitogenesis. Tbx6 interacts with proteins such as Wnt3a and RBPJ aligning its activity in these pathways to guide the formation of somites and establish anterior-posterior patterning.

Tbx6 mutations have been associated with congenital vertebral malformations and neural tube defects. These disorders arise due to the disruption of normal somitogenesis and mesoderm development often linked to faulty signaling in the Wnt and Notch pathways. Additionally protein interactions between Tbx6 and RBPJ a component of the Notch pathway highlight their collaborative role in ensuring proper vertebral column formation. Understanding Tbx6's involvement in these pathways provides insights into potential therapeutic targets for such developmental abnormalities.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells : neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia.

See full target information Tbx6

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 14:e4984 PubMed38737507

2024

Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Urs Kindler,Holm Zaehres,Lampros Mavrommatis

Developmental cell 58:2359-2375.e8 PubMed37647896

2023

Reconstructing human brown fat developmental trajectory in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Rao,Yannis Djeffal,Jerome Chal,Fabio Marchianò,Chih-Hao Wang,Ziad Al Tanoury,Svetlana Gapon,Alicia Mayeuf-Louchart,Ian Glass,Elizabeth M Sefton,Bianca Habermann,Gabrielle Kardon,Fiona M Watt,Yu-Hua Tseng,Olivier Pourquié

Nature communications 13:2325 PubMed35484123

2022

Periodic formation of epithelial somites from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Sanaki-Matsumiya,Mitsuhiro Matsuda,Nicola Gritti,Fumio Nakaki,James Sharpe,Vikas Trivedi,Miki Ebisuya

eLife 11: PubMed35088712

2022

Paraxial mesoderm organoids model development of human somites.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Budjan,Shichen Liu,Adrian Ranga,Senjuti Gayen,Olivier Pourquié,Sahand Hormoz

Stem cell reports 13:1083-1098 PubMed31708477

2019

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Bredenkamp,Jian Yang,James Clarke,Giuliano Giuseppe Stirparo,Ferdinand von Meyenn,Sabine Dietmann,Duncan Baker,Rosalind Drummond,Yongming Ren,Dongwei Li,Chuman Wu,Maria Rostovskaya,Sarah Eminli-Meissner,Austin Smith,Ge Guo

Stem cell reports 11:43-57 PubMed30008328

2018

Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Ran Wang,Zhisong He,Pierre Osteil,Emilie Wilkie,Xianfa Yang,Jun Chen,Guizhong Cui,Wenke Guo,Yingying Chen,Guangdun Peng,Patrick P L Tam,Naihe Jing

Nature protocols 11:1833-50 PubMed27583644

2016

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jérome Chal,Ziad Al Tanoury,Marie Hestin,Bénédicte Gobert,Suvi Aivio,Aurore Hick,Thomas Cherrier,Alexander P Nesmith,Kevin K Parker,Olivier Pourquié

PLoS biology 12:e1001937 PubMed25157815

2014

In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.

Applications

ICC/IF

Species

Mouse

Mina Gouti,Anestis Tsakiridis,Filip J Wymeersch,Yali Huang,Jens Kleinjung,Valerie Wilson,James Briscoe

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tbx6 抗体

Anti-Tbx6 antibody

Western blot - Anti-Tbx6 antibody (AB38883)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise