Anti-Tbx2 抗体 (ab33298)
Key features and details
- Rabbit polyclonal to Tbx2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tbx2 antibody
Tbx2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tbx2 -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Dog, Xenopus laevis
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Tbx2.
Immunogen の所有権に関して (Peptide available as ab35983.) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following Human tissue lysates: Kidney; Lung; Heart
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33298の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. -
組織特異性
Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary. -
配列類似性
Contains 1 T-box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 448779 Dog
- Entrez Gene: 6909 Human
- Entrez Gene: 21385 Mouse
- Omim: 600747 Human
- SwissProt: Q863A2 Dog
- SwissProt: Q13207 Human
- SwissProt: Q60707 Mouse
- Unigene: 531085 Human
see all -
別名
- FLJ10169 antibody
- HGNC:11597 antibody
- T box 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tbx2 antibody (ab33298)Formalin-fixed paraffin-embedded mouse bone marrow section stained with ab33298 Tbx2 antibody at 1/500 dilution.
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Western blot - Anti-Tbx2 antibody (ab33298)All lanes : Anti-Tbx2 antibody (ab33298) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 74 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab33298 は 6 報の論文で使用されています。
- McIntyre AJ et al. TBX2 acts as a potent transcriptional silencer of tumour suppressor genes through interaction with the CoREST complex to sustain the proliferation of breast cancers. Nucleic Acids Res 50:6154-6173 (2022). PubMed: 35687133
- Durbin AD et al. Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry. Nat Genet 50:1240-1246 (2018). PubMed: 30127528
- Cho GS et al. Tbx2 regulates anterior neural specification by repressing FGF signaling pathway. Dev Biol 421:183-193 (2017). PubMed: 27913219
- Lv Y et al. TBX2 over-expression promotes nasopharyngeal cancer cell proliferation and invasion. Oncotarget 8:52699-52707 (2017). PubMed: 28881763
- Liu F et al. TBX2 expression is regulated by PAX3 in the melanocyte lineage. Pigment Cell Melanoma Res 26:67-77 (2013). PubMed: 23020925
- Lüdtke TH et al. Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLoS Genet 9:e1003189 (2013). PubMed: 23341776